State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Wilmar (Shanghai) Biotechnology Research & Development Center Co. Ltd., Shanghai 200137, China.
J Agric Food Chem. 2021 Aug 18;69(32):9137-9146. doi: 10.1021/acs.jafc.0c05116. Epub 2020 Dec 18.
The digestion and absorption of different structural lipids in human milk may be different. Hence, by simulating in vitro infant digestion and Caco-2 cells to explore the effects of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL)/1,3-dilinoleoyl-2-palmitoylglycerol (LPL)/1,3-dioleoyl-2-palmitoylglycerol (OPO) and their mixtures (M) (OPL/LPL/OPO in M1, M2, and M3 were 1.5/0.5/1, 1.2/1.2/1, and 0.5/0.2/1, respectively) on digestion and absorption. The digestibility of the OPO group was higher than those of the OPL and LPL groups, and the M3 group was higher than the M1 and M2 groups. The synthesis and transport of triglycerides in Caco-2 cells in OPL and LPL groups were higher than the OPO group, and the M1 group was significantly higher than that of M3. The expression of FABP1, PPARα, and MTT protein in OPL and M1 groups was significantly higher than OPO and M3, respectively. There are differences in the digestion and absorption of differently structured lipids from this study.
人乳中不同结构脂质的消化吸收可能不同。因此，通过模拟体外婴儿消化和 Caco-2 细胞，探讨了 1-油酰基-2-棕榈酰基-3-亚油酸甘油酯（OPL）/1,3-二亚油酰基-2-棕榈酰甘油（LPL）/1,3-二油酰基-2-棕榈酰甘油（OPO）及其混合物（M）（M1、M2 和 M3 中 OPL/LPL/OPO 分别为 1.5/0.5/1、1.2/1.2/1 和 0.5/0.2/1）对消化和吸收的影响。OPO 组的消化率高于 OPL 和 LPL 组，M3 组高于 M1 和 M2 组。OPL 和 LPL 组 Caco-2 细胞中甘油三酯的合成和转运均高于 OPO 组，M1 组明显高于 M3 组。OPL 和 M1 组 FABP1、PPARα 和 MTT 蛋白的表达均明显高于 OPO 和 M3 组。不同结构脂质的消化吸收存在差异。
