Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Hubei Hongshan Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China.
Food Processing Research Institute, Anhui Engineering Laboratory of Agro-products Processing, School of Tea and Food Science, Anhui Agricultural University, No. 130 West Changjiang Road, Hefei, Anhui 230036, China.
Food Res Int. 2023 Jan;163:112168. doi: 10.1016/j.foodres.2022.112168. Epub 2022 Nov 26.
A novel and effective approach based on the two-step ethanolysis-esterification strategy was proposed for the controllable and simultaneous preparation of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL), 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) and 1,3-dilinoleoyl-2-palmitoyl-glycerol (LPL) with adjustable proportions. Enzymatic ethanolysis of fractionated palm stearin was carried out to yield 2-monopalmitoylglycerol (79.4 ± 0.6 %) with over 91.0 % purity at the optimal conditions. The immobilized Candida sp. lipase (CSL) on octyl-functionalized ordered mesoporous silica (OMS-C) was applied to re-esterify 2-monopalmitoylglycerol with oleic acid and linoleic acid for the simultaneous production of OPL, OPO, and LPL. The total content in the final products was 81.5 %, with 91.3 % of palmitic acid (PA) content at the sn-2 position. Besides, OPL/OPO/LPL was conveniently prepared with suitable proportions for worldwide infants by adjusting the ratio of acyl donors. This paper provides a novel and effective two-step ethanolysis-esterification strategy for the development of human milk fat substitutes (HMFS).
提出了一种新颖有效的两步乙醇解-酯化策略，可可控且同时制备具有可调比例的 1-油酰基-2-棕榈酰基-3-亚油酰基甘油（OPL）、1,3-二油酰基-2-棕榈酰基甘油（OPO）和 1,3-二亚油酰基-2-棕榈酰基甘油（LPL）。在最佳条件下，分馏棕榈硬脂通过酶促乙醇解可得到 91.0%以上纯度的 2-单棕榈酸甘油（79.4±0.6%）。固定化假丝酵母脂肪酶（CSL）接枝在辛基功能化的有序介孔硅（OMS-C）上，用于 2-单棕榈酸甘油与油酸和亚油酸重新酯化，同时生产 OPL、OPO 和 LPL。最终产物的总含量为 81.5%，sn-2 位的棕榈酸（PA）含量为 91.3%。此外，通过调整酰基供体的比例，方便地制备出具有合适比例的 OPL/OPO/LPL，适用于全球婴儿。本文为开发人乳脂肪替代品（HMFS）提供了一种新颖有效的两步乙醇解-酯化策略。
