Actin and calmodulin coexist in the equatorial segment of ejaculated boar sperm.

The direct overlay technique with iodinated calmodulin on boar sperm fractions evidenced a strong bond in the 45,000 molecular weight, which is the region recognized by anti-actin antibodies. This result and the close codistribution of the two staining patterns for calmodulin and actin with immunofluorescence and immunoelectronmicroscopy suggest a possible interaction between calmodulin and actin in boar sperm.