在家鸽（Columba livia）高、低精子活力睾丸中 microRNAs 和其靶 mRNAs 的表达分析。

Expression analysis of microRNAs and their target mRNAs of testes with high and low sperm motility in domestic pigeons (Columba livia).

机构信息

Animal Science College, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.

出版信息

Genomics. 2021 Jan;113(1 Pt 1):257-264. doi: 10.1016/j.ygeno.2020.12.024. Epub 2020 Dec 15.

DOI:10.1016/j.ygeno.2020.12.024

Abstract

Sperm motility is one of the most important indicators to evaluate poultry fertility. In order to explore key molecular regulation roles related to sperm motility, we employed testicular RNA sequencing of pigeon. A total of 705 known and 385 novel microRNAs were identified. Compared with the low sperm motility group, four upregulated and two downregulated miRNAs in the high sperm motility group were identified. A total of 3567 target mRNAs were predicted and four target mRNAs were selected to validate by qPCR. The miRNA-mRNA interaction network analysis, indicated that mmu-miR-183-5p /FOXO1 and PC-3p-244994_31/CHDH pairs might affect sperm motility. GO and KEGG annotation analysis showed that target genes of differentially expressed miRNAs were related to serine/threonine kinase activity, ATP binding, Wnt and MAPK signaling pathway. The study provided a global miRNAs transcriptome of pigeon and a novel insight into the expression of the miRNAs in testes that associated with sperm motility.

摘要

精子活力是评估家禽繁殖力的最重要指标之一。为了探讨与精子活力相关的关键分子调控作用，我们对鸽子的睾丸进行了 RNA 测序。共鉴定出 705 个已知和 385 个新的 microRNA。与低精子活力组相比，在高精子活力组中鉴定出四个上调和两个下调的 microRNA。共预测到 3567 个靶 mRNAs，并通过 qPCR 验证了四个靶 mRNAs。miRNA-mRNA 相互作用网络分析表明，mmu-miR-183-5p/FOXO1 和 PC-3p-244994_31/CHDH 对可能影响精子活力。GO 和 KEGG 注释分析表明，差异表达 microRNA 的靶基因与丝氨酸/苏氨酸激酶活性、ATP 结合、Wnt 和 MAPK 信号通路有关。该研究提供了鸽子全局 miRNAs 转录组图谱，并深入了解了与精子活力相关的睾丸中 miRNAs 的表达情况。