Universidad Autonoma de Nuevo Leon, Faculty of Medicine, Department of Psichiatry, Monterrey, Nuevo Leon, Mexico.
Universidad Autonoma de Nuevo Leon, Faculty of Medicine, Department of Immunology, Monterrey, Nuevo Leon, Mexico.
Cytokine. 2021 Feb;138:155400. doi: 10.1016/j.cyto.2020.155400. Epub 2020 Dec 15.
Influenza B virus (IBV) causes respiratory infectious disease. Cytokines are important immune mediators during infectious diseases. Cortisol and stress have been related to respiratory infection susceptibility and cytokine regulation. Little is known about systemic cytokines, cortisol, and perceived stress in the early stages of IBV infection. We researched the systemic cytokines and cortisol, as well as the perceived stress and blood cell count in patients infected with IBV. The diagnosis was established using the Luminex xTAG RVP kit and confirmed with qRT-PCR for IBV viral load. The perceived stress was evaluated using the perceived stress scale (PSS-10). Twenty-five plasma cytokines were determined using multiplex immunoassay and cortisol by ELISA. The leukocyte differential count was measured with a standard laboratory protocol. Th1, Th17, and IL-10 cytokines were higher in IBV infected patients (P < 0.05). Leukocytes and neutrophil count negatively correlated with viral load (P < 0.05). Perceived stress had a negative effect on monocyte and systemic cytokines in IBV infected patients (P < 0.05). Cortisol was higher in patients infected with IBV and correlated positively with CCL20 (P < 0.05). Cortisol showed a positive effect on most of the systemic cytokines (P < 0.05). In conclusion, a cytokine pattern was found in IBV infected patients, as well as the possible role of leukocyte counts in the control of IBV. Our results suggest the importance of cortisol and perceived stress on systemic cytokines in patients infected with IBV, but more studies are needed to understand their role in cytokine production in respiratory infectious disease.
乙型流感病毒（IBV）可引起呼吸道传染病。细胞因子是感染性疾病中重要的免疫调节剂。皮质醇和应激与呼吸道感染易感性和细胞因子调节有关。IBV 感染早期，系统性细胞因子、皮质醇和感知应激的研究较少。我们研究了 IBV 感染患者的系统性细胞因子和皮质醇，以及感知应激和血细计数。采用 Luminex xTAG RVP 试剂盒和 qRT-PCR 检测 IBV 病毒载量来确诊。采用感知应激量表（PSS-10）评估感知应激。采用多重免疫分析法测定 25 种血浆细胞因子，采用 ELISA 法测定皮质醇。采用标准实验室方案测量白细胞分类计数。IBV 感染患者 Th1、Th17 和 IL-10 细胞因子水平较高（P<0.05）。白细胞和中性粒细胞计数与病毒载量呈负相关（P<0.05）。感知应激对 IBV 感染患者单核细胞和系统性细胞因子有负面影响（P<0.05）。IBV 感染患者皮质醇水平较高，与 CCL20 呈正相关（P<0.05）。皮质醇对大多数系统性细胞因子有正向作用（P<0.05）。综上所述，我们发现了 IBV 感染患者的细胞因子模式，以及白细胞计数在控制 IBV 中的可能作用。我们的结果表明，皮质醇和感知应激对 IBV 感染患者系统性细胞因子的重要性，但需要更多研究来了解它们在呼吸感染性疾病中细胞因子产生中的作用。
