酵母缬氨酰-tRNA合成酶与tRNA相互作用的中子研究。
A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs.
作者信息
Giegé R, Jacrot B, Moras D, Thierry J C, Zaccai G
出版信息
Nucleic Acids Res. 1977 Jul;4(7):2421-7. doi: 10.1093/nar/4.7.2421.
Abstract
A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with its cognate and non cognate yeast tRNAs. It was shown that, when limited amounts of tRNA (either cognate or non cognate) are added to valyl-tRNA synthetase, a complex consisting of two enzyme molecules and one tRNA molecule is first formed. It is subsequently dissociated to a one to one complex when more tRNA is present in the solution. The association curve shows a maximum for a molecular ratio, enzyme over tRNA, equal to 2.
摘要
描述了一种利用溶液中的中子小角散射研究RNA-蛋白质复合物的新方法，并将其应用于酵母缬氨酰-tRNA合成酶与同源和非同源酵母tRNA相互作用的系统中。结果表明，当向缬氨酰-tRNA合成酶中添加少量tRNA（同源或非同源）时，首先形成由两个酶分子和一个tRNA分子组成的复合物。当溶液中存在更多tRNA时，该复合物随后解离为一对一的复合物。结合曲线显示，酶与tRNA的分子比等于2时出现最大值。
相似文献
1
A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs.酵母缬氨酰-tRNA合成酶与tRNA相互作用的中子研究。
Nucleic Acids Res. 1977 Jul;4(7):2421-7. doi: 10.1093/nar/4.7.2421.
2
Ultracentrifugation studies of yeast valyl-tRNA synthetase and of its interaction with tRNAVal.酵母缬氨酰 - tRNA合成酶及其与tRNAVal相互作用的超速离心研究。
Biochim Biophys Acta. 1978 Dec 21;521(2):597-605. doi: 10.1016/0005-2787(78)90301-5.
3
Influence of various factors on the recognition specificity of tRNAs by yeast valyl-tRNA synthetase.各种因素对酵母缬氨酰-tRNA合成酶识别tRNA特异性的影响。
Eur J Biochem. 1975 Oct 1;58(1):193-201. doi: 10.1111/j.1432-1033.1975.tb02364.x.
4
Interactions of yeast valyl-tRNA synthetase with RNAs and conformational changes of the enzyme.酵母缬氨酰 - tRNA合成酶与RNA的相互作用及该酶的构象变化
J Mol Biol. 1979 Apr 15;129(3):483-500. doi: 10.1016/0022-2836(79)90508-4.
5
Equilibrium measurements of cognate and noncognate interactions between aminoacyl transfer RNA synthetases and transfer RNA.氨酰基转移RNA合成酶与转移RNA之间同源和非同源相互作用的平衡测量。
Biochemistry. 1975 Jun 17;14(12):2775-80. doi: 10.1021/bi00683a034.
6
Effect of heavy water substitution for water on the tRNAVal-valyl-tRNA synthetase system from yeast.重水替代水对酵母中tRNAVal-缬氨酰-tRNA合成酶系统的影响。
Biochemistry. 1980 Jul 8;19(14):3158-64. doi: 10.1021/bi00555a007.
7
8
Valyl- and phenylalanyl-tRNA synthetase from baker's yeast: recognition of transfer RNA results from a multistep process, as indicated by inhibition of aminoacylation with modified transfer RNA.来自面包酵母的缬氨酰 - 和苯丙氨酰 - tRNA合成酶：tRNA的识别源于一个多步骤过程，如用修饰的tRNA抑制氨酰化所示。
Biochemistry. 1978 Oct 17;17(21):4509-14. doi: 10.1021/bi00614a023.
9
Contact areas of the turnip yellow mosaic virus tRNA-like structure interacting with yeast valyl-tRNA synthetase.芜菁黄花叶病毒类tRNA结构与酵母缬氨酰-tRNA合成酶相互作用的接触区域。
J Mol Biol. 1986 Sep 5;191(1):117-30. doi: 10.1016/0022-2836(86)90427-4.
10
The 32PPi--ATP isotope-exchange reaction catalyzed by the yeast valyl-tRNA synthetase: order of substrate binding and effect of tRNA.酵母缬氨酰 - tRNA合成酶催化的32PPi - ATP同位素交换反应：底物结合顺序及tRNA的影响
FEBS Lett. 1979 Jul 15;103(2):274-81. doi: 10.1016/0014-5793(79)81344-7.
引用本文的文献
1
Fifty years excitement with science: recollections with and without tRNA.与科学相伴的五十年激情：有与没有转运RNA的回忆
J Biol Chem. 2013 Mar 1;288(9):6679-87. doi: 10.1074/jbc.X113.453894. Epub 2013 Jan 16.
本文引用的文献
1
Structure of yeast phenylalanine tRNA at 3 A resolution.酵母苯丙氨酸tRNA在3埃分辨率下的结构
Nature. 1974 Aug 16;250(467):546-51. doi: 10.1038/250546a0.
2
Three-dimensional tertiary structure of yeast phenylalanine transfer RNA.酵母苯丙氨酸转移核糖核酸的三维三级结构。
Science. 1974 Aug 2;185(4149):435-40. doi: 10.1126/science.185.4149.435.
3
The crystal structure of tyrosyl-transfer RNA synthetase at 2-7 A resolution.分辨率为2-7埃的酪氨酰转运核糖核酸合成酶的晶体结构。
J Mol Biol. 1976 Aug 25;105(4):577-86. doi: 10.1016/0022-2836(76)90236-9.
4
Interpretation of tRNA-mischarging kinetics.tRNA错配动力学的解释
Eur J Biochem. 1976 Nov 1;70(1):147-58. doi: 10.1111/j.1432-1033.1976.tb10965.x.
5
Atomic coordinates and molecular conformation of yeast phenylalanyl tRNA. An independent investigation.酵母苯丙氨酰tRNA的原子坐标和分子构象。一项独立研究。
Nucleic Acids Res. 1976 Apr;3(4):1111-23. doi: 10.1093/nar/3.4.1111.
6
A low-resolution model of crystalline methionyl-transfer RNA synthetase from Escherichia coli.来自大肠杆菌的结晶甲硫氨酰 - 转移RNA合成酶的低分辨率模型。
J Mol Biol. 1976 Mar 25;102(1):93-101. doi: 10.1016/0022-2836(76)90075-9.
7
Influence of various factors on the recognition specificity of tRNAs by yeast valyl-tRNA synthetase.各种因素对酵母缬氨酰-tRNA合成酶识别tRNA特异性的影响。
Eur J Biochem. 1975 Oct 1;58(1):193-201. doi: 10.1111/j.1432-1033.1975.tb02364.x.
8
Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetase.两种形式的酵母缬氨酰 - tRNA合成酶的完全纯化及其结构和动力学性质研究
Biochimie. 1975;57(10):1167-76. doi: 10.1016/s0300-9084(76)80579-2.
9
The determination of stability constants from small-angle x-ray scattering data and the analysis of pH-dependent macromolecular equilibria.从小角X射线散射数据确定稳定性常数以及对pH依赖性大分子平衡的分析。
J Mol Biol. 1975 Dec 15;99(3):394-400. doi: 10.1016/s0022-2836(75)80134-3.
Zotero 插件