Stout C D, Mizuno H, Rubin J, Brennan T, Rao S T, Sundaralingam M
Nucleic Acids Res. 1976 Apr;3(4):1111-23. doi: 10.1093/nar/3.4.1111.
The atomic coordinates of yeast tRNA(Phe) in the monoclinic crystal form have been determined by an independent analysis from a model built into a 3 A MIR map. The overall molecular structure is found to be in agreement with those reported for the same crystal form by Ladner et al. (1975) and for the orthorhombic form by Quigley et al. (1975) and Kim et al. (1975). However, significant differences between any two of the four models are found in certain local regions of the molecule. The structure is analyzed in terms of the nucleotide stereochemistry and internucleotide phosphodiesters. A striking observation is that the majority of the nucleotide moieties occur in the conformation preferred by the constituent mononucleotides themselves. The internucleotide P-O bonds afford the primary source of flexibility for the folding of the polynucleotide backbone while the sugar pucker and C(4')-C(5') torsions provide the secondary source of flexibility.
通过对嵌入3埃MIR图谱的模型进行独立分析，已确定了单斜晶型酵母苯丙氨酸转运核糖核酸（tRNA(Phe)）的原子坐标。发现其整体分子结构与拉德纳等人（1975年）报道的相同晶型以及奎格利等人（1975年）和金等人（1975年）报道的正交晶型一致。然而，在分子的某些局部区域发现这四个模型中的任意两个之间存在显著差异。根据核苷酸立体化学和核苷酸间磷酸二酯对该结构进行了分析。一个引人注目的观察结果是，大多数核苷酸部分以组成单核苷酸自身所偏好的构象出现。核苷酸间的P-O键为多核苷酸主链的折叠提供了主要的灵活性来源，而糖的构象和C(4')-C(5')扭转提供了次要的灵活性来源。
