大肠杆菌K-12中外切核酸酶III结构基因xthA的遗传定位。
Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.
作者信息
White B J, Hochhauser S J, Cintron N M, Weiss B
出版信息
J Bacteriol. 1976 Jun;126(3):1082-8. doi: 10.1128/jb.126.3.1082-1088.1976.
Abstract
The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB-xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.
摘要
通过用噬菌体P1进行两因子和三因子转导杂交，确定了xthA、pncA和pabB基因相对于其他基因的顺序。所研究的基因按顺时针顺序pheS-pfkB-xthA-pncA-gap-pabB-fadD跨越大肠杆菌K-12遗传图谱的2分钟（2%）。检查了11个独立获得的xth突变；所有这些突变都已知会影响核酸外切酶III及其相关的核酸内切酶II活性，并且所有突变都定位在xthA区域。发现pncA突变赋予对6-氨基烟酰胺的抗性，而一些pheS突变已知赋予对对氟苯丙氨酸的抗性。通过选择这些共转导的耐药标记，xth突变很容易转移到其他菌株中。
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