Laboratorio de Endocrinología y Metabolismo Animal, Universidad de la República, Montevideo, Uruguay.
Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL, United States.
Theriogenology. 2021 Feb;161:210-218. doi: 10.1016/j.theriogenology.2020.12.008. Epub 2020 Dec 10.
Transcriptomics and bioinformatics were used to investigate the potential interactions of undernutrition and the presence of the conceptus at the time of maternal recognition of pregnancy on uterine immune system and remodeling. Adult Rasa Aragonesa ewes were allocated to one of two planes of nutrition for 28 days: maintenance energy intake (control; 5 cyclic, 6 pregnant ewes) providing 7.8 MJ of metabolisable energy and 0.5 maintenance intake (undernourished; 6 cyclic, 7 pregnant ewes) providing 3.9 MJ of metabolisable energy per ewe. Uterine gene expression was measured using Agilent 15 K Sheep Microarray chip on day 14 of estrus or pregnancy. Functional bioinformatics analyses were performed using PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System. Pregnancy affected the expression of 18 genes in both control and undernourished ewes, underscoring the relevance for embryo-maternal interactions. Immune system evidenced by classical interferon stimulated genes were activated in control and -in a lesser extent-in undernourished pregnant vs cyclic ewes. Genes involved in uterine remodeling such as protein metabolism were also upregulated with the presence of an embryo in control and undernourished ewes. However, relevant genes for the adaptation of the uterus to the embryo were differentially expressed between pregnant vs cyclic ewes both in control and undernourished groups. Undernutrition alone led to an overall weak activation of immune system pathways both in cyclic and pregnant ewes. Data revealed that cellular and immune adaptations of the uterus to pregnancy are dependent on the nutritional status.
采用转录组学和生物信息学方法研究了营养不良和胚泡存在与母体妊娠识别时对子宫免疫系统和重塑的潜在相互作用。将成年拉沙洛萨（Rasa Aragonesa）母羊分为两种营养水平组，分别进行 28 天的饲养：维持能量摄入（对照组；5 只发情，6 只妊娠母羊）提供 7.8MJ 代谢能，以及低营养摄入（营养不良组；6 只发情，7 只妊娠母羊）提供 3.9MJ 代谢能。发情或妊娠第 14 天使用安捷伦（Agilent）15K 绵羊微阵列芯片测量子宫基因表达。使用 PANTHER（蛋白分析通过进化关系）分类系统进行功能生物信息学分析。妊娠影响了对照组和低营养组母羊中 18 个基因的表达，突出了胚胎与母体相互作用的重要性。免疫系统表现为经典的干扰素刺激基因在对照组和低营养妊娠母羊中被激活，但在低营养妊娠母羊中的激活程度较低。与胚胎存在相关的子宫重塑相关基因，如蛋白质代谢，也在对照组和低营养组的妊娠母羊中上调。然而，与胚胎适应子宫相关的重要基因在对照组和低营养组的妊娠与发情母羊之间的表达存在差异。单独的低营养导致在发情和妊娠母羊中免疫系统途径的整体激活较弱。研究结果表明，子宫对妊娠的细胞和免疫适应取决于营养状况。
