de Brun Victoria, Meikle Ana, Fernández-Foren Andrea, Forcada Fernando, Palacín Inmaculada, Menchaca Alejo, Sosa Cecilia, Abecia José-Alfonso
Laboratorio de Técnicas Nucleares, Universidad de la República, Uruguay.
Laboratorio de Técnicas Nucleares, Universidad de la República, Uruguay.
Anim Reprod Sci. 2016 Oct;173:80-6. doi: 10.1016/j.anireprosci.2016.08.016. Epub 2016 Aug 31.
Embryos from undernourished and control donor ewes were transferred to undernourished and control recipient ewes. Progesterone and metabolic hormones were investigated in recipient ewes to determine their association with pregnancy success. Forty-five donor and 52 recipient Rasa Aragonesa ewes were fed 1.5 (control group; donor n=20; recipient n=25) or 0.5 (low group; donor n=25; recipient n=27) times the daily requirements for maintenance from the onset of estrous synchronization treatment to embryo collection and transfer. The embryos were collected 7days after the onset of estrus (day 0), and two good-quality embryos were transferred into each recipient ewe. The percentage of pregnant ewes on day 18 and 40 did not differ between the two groups, although the recipient undernourished ewes tended to have greater late embryonic mortality (from days 18-40) than the control recipient ewes (P=0.11). No effect of the nutrition of the donor was found. Recipients that became pregnant had a higher ovulation rate than non-pregnant ewes (P=0.02). Undernourished ewes had lower plasma insulin concentrations than control ewes (P=0.03), and those that suffered late embryo mortality (from days 18-40) tended to have lower insulin and progesterone concentrations than their counterparts that remained pregnant (P=0.06 and P=0.07, respectively). In this study, pregnancy in control and undernourished recipient ewes was not associated with the origin of the embryo (undernourished and control donors). In conclusion, failure to establish and maintain a pregnancy was associated with lower progesterone and insulin levels one week after estrus in recipient ewes.
将营养不良供体母羊和对照供体母羊的胚胎移植到营养不良受体母羊和对照受体母羊体内。对受体母羊的孕酮和代谢激素进行了研究，以确定它们与妊娠成功的关联。45只供体和52只受体阿拉贡萨母羊从发情同步处理开始到胚胎采集和移植期间，按照维持每日需求量的1.5倍（对照组；供体n = 20；受体n = 25）或0.5倍（低营养组；供体n = 25；受体n = 27）进行饲养。在发情开始后7天（第0天）采集胚胎，并将两个优质胚胎移植到每只受体母羊体内。两组在第18天和第40天的妊娠母羊百分比没有差异，尽管营养不良的受体母羊晚期胚胎死亡率（第18 - 40天）往往高于对照受体母羊（P = 0.11）。未发现供体营养状况的影响。怀孕的受体母羊排卵率高于未怀孕的母羊（P = 0.02）。营养不良的母羊血浆胰岛素浓度低于对照母羊（P = 0.03），且那些发生晚期胚胎死亡（第18 - 40天）的母羊胰岛素和孕酮浓度往往低于其仍保持妊娠的对应母羊（分别为P = 0.06和P = 0.07）。在本研究中，对照和营养不良受体母羊的妊娠与胚胎来源（营养不良和对照供体）无关。总之，受体母羊发情一周后孕酮和胰岛素水平较低与妊娠建立和维持失败有关。
