College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China; Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, China.
College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China.
Phytochemistry. 2021 Mar;183:112611. doi: 10.1016/j.phytochem.2020.112611. Epub 2020 Dec 17.
Magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones in a single rapeseed seed was developed by using ultra-high performance liquid chromatography-tandem mass spectrometry. The FeO@TiC@β-cyclodextrin nanoparticles were firstly synthesized and used as an adsorbent for the solid-phase extraction of phytohormones. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Satisfactory methodological performance was achieved by optimization of the parameters. Linearities (R > 0.9928) and recoveries (80.4 %-115.1%) at three spiked levels, as well as the low matrix effect (from -16.63% to 17.06%) and limits of detection (0.89-13.62 pg/mL) were obtained. The spatio-temporal profiling of target phytohormones in different tissues of rapeseed germination was investigated. This method was successfully employed for analyzing target phytohormones in different oilseeds samples.
采用超高效液相色谱-串联质谱法，建立了一种在单个油菜籽中同时分析 12 种植物激素的磁固相萃取-原位衍生方法。首先合成了 FeO@TiC@β-环糊精纳米粒子，并将其用作植物激素的固相萃取吸附剂。巧妙地将磁分散固相萃取和通过添加 N-(3-二甲氨基丙基)-N'-乙基碳二亚胺的原位衍生结合在一起。这种高效的预处理方法将提取、净化和衍生过程集成到一个步骤中。通过优化参数，获得了令人满意的方法性能。在三个加标水平下均获得了良好的线性（R>0.9928）和回收率（80.4%-115.1%），以及低的基质效应（-16.63%至 17.06%）和检测限（0.89-13.62 pg/mL）。研究了油菜籽萌发过程中不同组织中目标植物激素的时空分布情况。该方法成功用于分析不同油料样品中的目标植物激素。
