Shi Nian, Liu Yuwei, Li Wenxuan, Yan Shumei, Ma Lei, Xu Xia, Chen Di
Physics Diagnostic Division, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases of Henan Province, and School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Food Chem X. 2023 Dec 21;21:101090. doi: 10.1016/j.fochx.2023.101090. eCollection 2024 Mar 30.
Consuming foods with excess sulfonamide residues threatens human health, underscoring the importance of their detection in food. This study presents an innovative one-pot derivatization/magnetic solid-phase extraction (OPD/MSPE) method for sulfonamides analysis. This approach integrates the derivatization and extraction steps into a single process. The sample solution, along with the derivatization reagent fluorescamine and the sorbent magnetic hydroxyl multi-walled carbon nanotubes, is mixed and vortexed for 3 min. This procedure simultaneously conducts derivatization and extraction, with easy phase separation using an external magnet. This streamlined sample preparation method is completed in only 5 min and, when combined with liquid chromatography-fluorescence detection (LC-FLD), demonstrates excellent linearity (R > 0.99) and satisfactory detection limits (0.004-0.04 ng/g) for the quantification of nine sulfonamides in honey samples. The proposed OPD/MSPE-LC-FLD method is distinguished by its simplicity, rapidity, high sensitivity, and specificity, making it an outstanding advancement in the field of food safety analysis.
食用含有过量磺胺类药物残留的食品会威胁人类健康，这凸显了在食品中检测它们的重要性。本研究提出了一种用于磺胺类药物分析的创新型一锅法衍生化/磁性固相萃取（OPD/MSPE）方法。该方法将衍生化和萃取步骤整合为一个单一过程。将样品溶液与衍生化试剂荧光胺和吸附剂磁性羟基多壁碳纳米管混合并涡旋3分钟。此过程同时进行衍生化和萃取，使用外部磁铁即可轻松实现相分离。这种简化的样品制备方法仅需5分钟即可完成，并且与液相色谱 - 荧光检测（LC - FLD）相结合时，在蜂蜜样品中九种磺胺类药物的定量分析方面显示出出色的线性（R>0.99）和令人满意的检测限（0.004 - 0.04 ng/g）。所提出的OPD/MSPE - LC - FLD方法具有简单、快速、高灵敏度和特异性的特点，使其成为食品安全分析领域的一项杰出进展。
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