Department of Medical Physics and Physiology, School of Medical Sciences, Arak University of Medical Science, Arak, Iran.
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
J Cancer Res Ther. 2020 Oct-Dec;16(6):1250-1257. doi: 10.4103/jcrt.JCRT_839_16.
We want to compare the synergistic effect of low-intensity, dual-frequency (dual) ultrasound (US), applied in a repetitive sonication mode, and acoustically active lipospheres (AALs) containing doxorubicin (DOX) in a murine model (Balb/C).
The tumor-bearing mice were divided into nine groups, namely two untreated groups (control and sham), and seven experimental groups, including treated with dual-frequency US (150 kHz + 1MHz), triple exposure (3×30min) dual-frequency US, DOX (2 mg/kg intravenous), DOX in combination with single exposure (30 min) to dual-frequency US (drug + dual), DOX in combination with triple (3 × 30 min) exposure to dual-frequency US (drug + dual [REP]), AALs containing the drug-loaded (AAL), and a group receiving AAL in combination with single exposure (30 min) dual-frequency US (AAL + dual), respectively.
The effectiveness of DOX on tumor growth was enhanced by a factor of three when combined with the triple exposures of dual US (drug + dual [REP]). This combination protocol further increased the times needed for each tumor to 2 and 7 times its initial volume, respectively by 94% and 36% compared to the drug group. During the 30 days, following the treatment of tumors, the relative volume of tumors in AAL group was 118% less than that of the drug group. The survival rate of the groups treated with drug and AAL + dual was increased by 78.7% and 167% compared with sham, respectively.
Although as a short treatment, a major improvement in treatment was observed by (drug + dual [REP]) compared with other treatments, the AAL + dual treatment compared with (drug + dual [REP]) showed an increase in the survival rates, hence more preferable over long periods.
我们希望在 Balb/C 小鼠模型中比较低强度双频（双）超声（US）的协同效应，该超声以重复超声模式应用，并与含有阿霉素（DOX）的声敏脂质体（AAL）联合使用。
荷瘤小鼠分为 9 组，即两组未处理组（对照和假处理）和 7 组实验组，分别用双频 US（150 kHz+1MHz）、三次（3×30min）双频 US 处理、DOX（2mg/kg 静脉内）、DOX 联合单次（30min）双频 US（药物+双）、DOX 联合三次（3×30min）双频 US（药物+双[REP]）、载药 AAL 组和单次（30min）双频 US 联合 AAL 组（AAL+双）处理。
DOX 与三次双频 US （药物+双[REP]）联合使用时，肿瘤生长的效果增强了三倍。与药物组相比，该联合方案使每个肿瘤的倍增时间分别增加了 2 倍和 7 倍，达到初始体积的 94%和 36%。在治疗后 30 天，AAL 组肿瘤的相对体积比药物组小 118%。与假处理组相比，药物组和 AAL+双组的存活率分别提高了 78.7%和 167%。
虽然（药物+双[REP]）作为一种短期治疗方法与其他治疗方法相比有明显改善，但与（药物+双[REP]）相比，AAL+双治疗方法提高了存活率，因此在长期内更可取。
