Liu Jinxiang, Li Zan, Wang Yujue, Zhang Quanqi
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Front Physiol. 2020 Dec 4;11:612036. doi: 10.3389/fphys.2020.612036. eCollection 2020.
Ringer's or phosphate buffer saline (PBS) solution buffer usually was used as dilution butter in intraperitoneal injection. Stress could activate immune response, inflammatory response and glycogen metabolic process. The impact of solution buffer as a stressor to immune system was ignored in immunostimulatory experiment. In this report, we tested the hypothesis that the innate immune response and glycogen metabolic process were altered when it were challenged with Ringer's in Japanese flounder (). RNA-seq was performed after challenge with Ringer's at 8 h and 48 h. The data revealed that the expression profiles of blood, gill, and kidney were significantly changed. Differentially expressed genes (DEGs) were identified, and energy metabolic and immune-related genes were up-regulated or down-regulated obviously. GO and KEGG analyses showed that DEGs were mainly enriched in innate immune terms and pathways. Weighted gene co-expression networks analysis (WGCNA) also indicated the highest association module with stress. A total of 16 genes were detected in the gray module, which were immune-related and metabolic-related genes. These results provided fundamental information on intraperitoneal injection with solution buffer. It offered useful clues to further explore the functional mechanism of stress and immunity.
林格氏液或磷酸盐缓冲盐水（PBS）溶液缓冲液通常在腹腔注射中用作稀释液。应激可激活免疫反应、炎症反应和糖原代谢过程。在免疫刺激实验中，溶液缓冲液作为应激源对免疫系统的影响被忽视了。在本报告中，我们检验了一个假设，即当用林格氏液刺激日本牙鲆时，其先天免疫反应和糖原代谢过程会发生改变。在接受林格氏液刺激8小时和48小时后进行RNA测序。数据显示，血液、鳃和肾脏的表达谱发生了显著变化。鉴定出差异表达基因（DEG），能量代谢和免疫相关基因明显上调或下调。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，DEG主要富集于先天免疫术语和途径。加权基因共表达网络分析（WGCNA）也表明与应激关联度最高的模块。在灰色模块中总共检测到16个基因，它们是免疫相关和代谢相关基因。这些结果提供了关于腹腔注射溶液缓冲液的基础信息。为进一步探索应激和免疫的功能机制提供了有用线索。
