Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Shandong Province, 266200, PR China.
Fish Shellfish Immunol. 2018 Sep;80:133-140. doi: 10.1016/j.fsi.2018.05.038. Epub 2018 Jun 1.
Water temperature fluctuations are considered to be a major factor affecting the immune functions and metabolic processes of scallops. To better understand the immune defense mechanisms of Yesso scallop, Patinopecten yessoensis following exposure to water temperature fluctuations, transcriptomic profiles in the gills from high-frequency fluctuations (HF_G), low-frequency fluctuations (LF_G), and no fluctuations (NF_G) groups were obtained using HiSeq™ 2500 (Illumina). For HF_G, scallops were transferred directly between 18 and 8 °C every 4 h and for 10 fluctuations, while scallops in LF_G were transferred between 18 and 13 °C every 12 h, for a total of 4 fluctuations. A total of 442,922,590 clean reads were generated in 9 libraries and then assembled into 210,780 unigenes with an average length of 705 bp and an N50 of 1253 bp. Based on sequence similarity, 54,529 unigenes (25.87%) were annotated in at least one database. Comparative analysis revealed that 696 unigenes differentially expressed in temperature stressed groups compared with the control, including 229 unigenes between HF_G and NF_G, and 548 unigenes between LF_G and NF_G, respectively. Additionally, among these differentially expressed genes (DEGs), there were 41 immune-related unigenes and 16 protein metabolism-related unigenes. These results provide fundamental information on the molecular defense mechanisms in the Yesso scallop gills after exposure to water temperature fluctuations.
水温波动被认为是影响扇贝免疫功能和代谢过程的主要因素。为了更好地了解虾夷扇贝（Patinopecten yessoensis）在经历水温波动后的免疫防御机制，我们使用 HiSeq™ 2500（Illumina）获得了来自高频波动（HF_G）、低频波动（LF_G）和无波动（NF_G）组的鳃转录组图谱。对于 HF_G，扇贝每 4 小时直接从 18°C 转移到 8°C，共进行了 10 次波动，而 LF_G 中的扇贝每 12 小时从 18°C 转移到 13°C，共进行了 4 次波动。9 个文库共产生了 442,922,590 条清洁读数，然后组装成 210,780 条 unigenes，平均长度为 705 bp，N50 为 1253 bp。基于序列相似性，54,529 条 unigenes（25.87%）在至少一个数据库中被注释。比较分析显示，与对照组相比，在温度胁迫组中有 696 条差异表达的 unigenes，包括 HF_G 和 NF_G 之间的 229 条 unigenes，以及 LF_G 和 NF_G 之间的 548 条 unigenes。此外，在这些差异表达基因（DEGs）中，有 41 条与免疫相关的 unigenes 和 16 条与蛋白质代谢相关的 unigenes。这些结果为虾夷扇贝鳃在经历水温波动后的分子防御机制提供了基础信息。
