Ohno Katie M, Bishell Amy B, Stanosz Glen R
USDA Forest Service, Forest Products Laboratory, Madison, WI, United States.
Department of Forestry and Wildlife Ecology, University of Wisconsin-Madison, Madison, WI, United States.
Front Microbiol. 2020 Dec 4;11:586940. doi: 10.3389/fmicb.2020.586940. eCollection 2020.
Copper tolerance of brown-rot basidiomycete decay fungi can lessen the efficacy of copper-containing wood preservatives for wood products in-service. The purpose of this study was to evaluate wood mass loss and differential expression of three genes that have putative annotations for copper-transporting ATPase pumps (FIBRA_00974, FIBRA_04716, and FIBRA_01430). Untreated southern pine (SP) and SP treated with three concentrations of ammoniacal copper citrate (CC, 0.6, 1.2, and 2.4%) were exposed to two copper-tolerant isolates (FP-90848-T and L-9414-SP) and copper-sensitive isolate (MAD 617) in a 4-week-long standard decay test (AWPA E10-19). Decay of copper-treated wood was inhibited by ( = 0.001); however, there was no inhibition of decay with increasing copper concentrations by both isolates. Initially, and one isolate (L-9414-SP) highly upregulated FIBRA_00974 and FIBRA_04716 on copper-treated wood at week 1 ( = 0.005), but subsequent expression was either not detected or was similar to expression on untreated wood ( = 0.471). The other isolate (FP-90848-T) downregulated FIBRA_00974 ( = 0.301) and FIBRA_04716 ( = 0.004) on copper-treated wood. FIBRA_01430 expression by was not detected, but was upregulated by both FP-90848-T ( = 0.481) and L-9414-SP ( = 0.392). Results from this study suggest that all three test fungi utilized different mechanisms when decaying copper-treated wood. Additionally, results from this study do not provide support for the involvement of these putative gene annotations for copper-transporting ATPase pumps in the mechanism of copper-tolerance.
褐腐担子菌腐朽真菌对铜的耐受性会降低含铜木材防腐剂在使用中对木制品的防腐效果。本研究的目的是评估木材质量损失以及三个具有假定铜转运ATP酶泵注释的基因（FIBRA_00974、FIBRA_04716和FIBRA_01430）的差异表达。未处理的南方松（SP）以及用三种浓度的氨性柠檬酸铜（CC，0.6%、1.2%和2.4%）处理过的SP，在为期4周的标准腐朽试验（AWPA E10 - 19）中，暴露于两种耐铜分离株（FP - 90848 - T和L - 9414 - SP）以及铜敏感分离株（MAD 617）。铜处理木材的腐朽受到抑制（ = 0.001）；然而，两种耐铜分离株均未随着铜浓度增加而抑制腐朽。最初，一种耐铜分离株（L - 9414 - SP）在第1周时在铜处理木材上使FIBRA_00974和FIBRA_04716高度上调（ = 0.005），但随后未检测到表达，或与未处理木材上的表达相似（ = 0.471）。另一种耐铜分离株（FP - 90848 - T）在铜处理木材上使FIBRA_00974（ = 0.301）和FIBRA_04716（ = 0.004）下调。未检测到耐铜分离株对FIBRA_01430的表达，但FP - 90848 - T（ = 0.481）和L - 9414 - SP（ = 0.392）均使其上调。本研究结果表明，所有三种测试真菌在腐朽铜处理木材时采用了不同机制。此外，本研究结果不支持这些假定的铜转运ATP酶泵基因注释参与耐铜机制。
