Suleiman Mohamed H A, Ateeg Ali A
Department of Chemistry, College of Science, King Khalid University, Abha 61413, Asir, Saudi Arabia.
Department of Chemistry, Faculty of Science, University of Kordofan, El Obeid, Sudan.
Evid Based Complement Alternat Med. 2020 Dec 3;2020:6690433. doi: 10.1155/2020/6690433. eCollection 2020.
is commonly used in traditional medicine to treat gastrointestinal disorders and diabetes. In this study, aqueous ethanol (AE) and aqueous methanol (AM) extracts from aerial parts and roots of were investigated. The total phenolic (TPC) and flavonoid (TFC) contents and antioxidant capacities in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydrogen peroxide (HO), and ferric reducing antioxidant power (FRAP) assays were determined, and the correlations among the results were assessed using Pearson's correlation. The antimicrobial activity was assessed through agar disc diffusion and broth microdilution assays. Phytochemical screening showed that extracts had alkaloids, glycosides, saponins, triterpenoids, phenols, and flavonoids in different abundances. The aerial part-AE extract contained low TPC (30.17 ± 4.24 mg GAE/g) and TFC (7.40 ± 1.02 mg QE/g) and displayed significant antioxidant capacity in the DPPH (IC = 52.17 ± 7.30 g/mL), HO (91.22 ± 2.60 g/mL), and FRAP (EC = 98.70 ± 2.21 g/mL) assays. By contrast, the root-AM extract contained high amounts of TPC (87.72 ± 7.75 mg GAE/g) and TFC (25.60 ± 1.57 mg QE/g). It showed significantly high antioxidant activity with IC values of 12.33 ± 1.88 g/mL in the DPPH and 39.37 ± 2.59 g/mL in the HO assays, as well as reducing power capacity with an EC value of 20.82 ± 1.14 g/mL in the FRAP assay. Both TPC and TFC were exhibited negative correlations ( < 0.01) with the IC and EC values obtained in the applied antioxidant assays. The aerial part-AM extract showed the highest inhibitory activity against (26.5 ± 0.20 mm), followed by (19.4 ± 0.40 mm) and (17.7 ± 0.49 mm). . was the most affected microorganism with a minimum inhibitory concentration of 128 g/mL against the aerial part-AM extract. Interestingly, all evaluated extracts showed potent antifungal activity against . However, aerial part-AM was the most effective, with an inhibition zone of 12.6 ± 0.17 mm. The results concluded that possesses different phytochemicals displaying significant antioxidant and antimicrobial activities, thus lending credence to its use in traditional medicine.
常用于传统医学中治疗胃肠道疾病和糖尿病。在本研究中,对[植物名称]地上部分和根部的水乙醇(AE)提取物和水甲醇(AM)提取物进行了研究。测定了总酚(TPC)和总黄酮(TFC)含量以及1,1 - 二苯基 - 2 - 苦基肼(DPPH)清除、过氧化氢(HO)和铁还原抗氧化能力(FRAP)测定的抗氧化能力,并使用Pearson相关性评估结果之间的相关性。通过琼脂平板扩散法和肉汤微量稀释法评估抗菌活性。植物化学筛选表明,[植物名称]提取物含有不同含量的生物碱、糖苷、皂苷、三萜类、酚类和黄酮类。地上部分 - AE提取物的TPC含量低(30.17±4.24mg GAE/g),TFC含量低(7.40±1.02mg QE/g),在DPPH(IC = 52.17±7.30μg/mL)、HO(91.22±2.60μg/mL)和FRAP(EC = 98.70±2.21μg/mL)测定中表现出显著的抗氧化能力。相比之下,根部 - AM提取物含有大量的TPC(87.72±7.75mg GAE/g)和TFC(25.60±1.57mg QE/g)。在DPPH测定中其IC值为12.33±1.88μg/mL,在HO测定中为39.37±2.59μg/mL,显示出显著的高抗氧化活性,在FRAP测定中其EC值为2 .82±1.14μg/mL,具有还原能力。TPC和TFC与应用抗氧化测定中获得的IC和EC值均呈负相关(<0.01)。地上部分 - AM提取物对[微生物名称1]的抑制活性最高(26.5±0.20mm),其次是[微生物名称2](19.4±0.40mm)和[微生物名称3](17.7±0.49mm)。[微生物名称4]是受影响最大的微生物,对地上部分 - AM提取物的最低抑菌浓度为128μg/mL。有趣的是,所有评估的提取物对[真菌名称]均显示出强效的抗真菌活性。然而,地上部分 - AM最有效,抑菌圈为12.6±0.17mm。结果表明,[植物名称]具有不同的植物化学物质,表现出显著的抗氧化和抗菌活性,因此证实了其在传统医学中的应用。