基于多个数据集的 Chromobox 家族成员在胃癌中的作用鉴定。

Identification of the Roles of Chromobox Family Members in Gastric Cancer: A Study Based on Multiple Datasets.

机构信息

The Second Xiangya Hospital, Central South University, 139th Renmin middle Road, Changsha, Hunan, China.

Department of Surgery, Hanyang Hospital, Wuhan University of Science and Technology, No. 53, Moshuihu Road, Wuhan, Hubei, China.

出版信息

Biomed Res Int. 2020 Nov 5;2020:5306509. doi: 10.1155/2020/5306509. eCollection 2020.

DOI:10.1155/2020/5306509

PMID:33344640

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7732380/

Abstract

BACKGROUND

As the important components in polycomb repressive complexes 1 (PRC1) and heterochromatin protein 1 (HP1), Chromobox (CBX) family members are involved in epigenetic regulatory function, transcriptional repression, and other cellular metabolisms. Increasing studies have indicated significant associations between CBX and tumorigenesis, which is a progression in different types of cancers. However, the information about the roles of each CBX in gastric cancer is extremely limited.

METHODS

We explored CBX mRNA expression, corrections with clinicopathological parameters, protein expression, prognostic values, enrichment analysis with several databases including Oncomine, Human Protein Atlas, UALCAN, Kaplan-Meier plotter, cBioPortal, GeneMANIA, and Enrichr.

RESULTS

In our study, comparing to the normal tissues, higher mRNA expression of CBX1/2/3/4/5/8 and lower mRNA expression of CBX7 were found in GC tissues while upregulations of CBX1/2/3/4/5/8 and downregulations of CBX7 were indicated to be significantly correlated to the nodal metastasis status and individual cancer stages in GC patients. As for protein level, the expression of CBX2/3/4/5/6 was higher and the expression of CBX7 was lower in the GC tissues than those in the normal. What is more, higher mRNA expression of CBX1/5/6/8 and lower mRNA expression of CBX7 were markedly correlated to poor outcomes of OS and FP in GC patients. Besides, high mutation rate of CBXs (42%) was observed in GC patients.

CONCLUSIONS

We suggest that CBX5/7 may serve as potential therapeutic targets for GC while CBX1/8 may serve as potential prognostic indicators for GC.

摘要

背景

作为多梳抑制复合物 1（PRC1）和异染色质蛋白 1（HP1）的重要组成部分，Chromobox（CBX）家族成员参与表观遗传调控功能、转录抑制和其他细胞代谢。越来越多的研究表明 CBX 与肿瘤发生之间存在显著关联，这是不同类型癌症的一个进展过程。然而，关于每个 CBX 在胃癌中的作用的信息极其有限。

方法

我们研究了 CBX mRNA 表达、与临床病理参数的相关性、蛋白表达、预后价值，并利用 Oncomine、Human Protein Atlas、UALCAN、Kaplan-Meier plotter、cBioPortal、GeneMANIA 和 Enrichr 等数据库进行了富集分析。

结果

在我们的研究中，与正常组织相比，GC 组织中 CBX1/2/3/4/5/8 的 mRNA 表达较高，而 CBX7 的 mRNA 表达较低，且 CBX1/2/3/4/5/8 的上调和 CBX7 的下调与 GC 患者的淋巴结转移状态和个体癌症分期显著相关。在蛋白水平上，GC 组织中 CBX2/3/4/5/6 的表达较高，CBX7 的表达较低。此外，CBX1/5/6/8 的高 mRNA 表达和 CBX7 的低 mRNA 表达与 GC 患者的 OS 和 FP 预后不良显著相关。此外，GC 患者的 CBX 突变率较高（42%）。