Guo Jinxing, Zhang Zichao, Pan Lijie, Zhou Yuanhang
1 Department of General Surgery, Renhe Hospital, Baoshan District, Shanghai, China.
2 Department of General Surgery, Digestive Medical Center, The First Affiliated Hospital of Tsinghua University, Jiuxianqiao, Chaoyang District, Beijing, China.
Technol Cancer Res Treat. 2018 Jan 1;17:1533033818816061. doi: 10.1177/1533033818816061.
Gastric cancer is one of the most frequently diagnosed cancer types in China and also the leading causes of cancer-related death. Previous study showed chromobox 5 expression was elevated in gastric cancer, but little is known regarding the precise molecular mechanisms by which chromobox 5 expression was modulated. In this study, we revealed that chromobox 5 could promote gastric cancer cell proliferation, migration, and invasion in vitro. We screened and identified microRNA-758-3p, whose expression was downregulated in gastric cancer tissues and cell lines, which was a potential upstream molecule of chromobox 5. Upregulation of microRNA-758-3p could markedly downregulate the expression of chromobox 5. Additionally, expression of microRNA-758-3p and chromobox 5 was inversely correlated in gastric cancer tissues. Moreover, microRNA-758-3p overexpression suppressed gastric cancer cell proliferation, migration, and invasion, but these effects can be partially reversed by chromobox 5 overexpression. Collectively, our results indicate that microRNA-758-3p serves as a tumor suppressor and plays a crucial role in inhibiting the proliferation, migration, and invasion of gastric cancer via targeting chromobox 5 and implicate its potential application in cancer therapy.
胃癌是中国最常被诊断出的癌症类型之一，也是癌症相关死亡的主要原因。先前的研究表明，染色体框蛋白5（CBX5）在胃癌中表达升高，但关于CBX5表达调控的确切分子机制知之甚少。在本研究中，我们发现CBX5可促进胃癌细胞在体外的增殖、迁移和侵袭。我们筛选并鉴定出微小RNA-758-3p（miR-758-3p），其在胃癌组织和细胞系中表达下调，是CBX5的潜在上游分子。miR-758-3p的上调可显著下调CBX5的表达。此外，miR-758-3p与CBX5在胃癌组织中的表达呈负相关。而且，miR-758-3p过表达抑制胃癌细胞的增殖、迁移和侵袭，但这些作用可被CBX5过表达部分逆转。总体而言，我们的结果表明，miR-758-3p作为一种肿瘤抑制因子，通过靶向CBX5在抑制胃癌的增殖、迁移和侵袭中起关键作用，并暗示了其在癌症治疗中的潜在应用。
