Xue Jiajia, Wu Tong, Qiu Jichuan, Xia Younan
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; School of Chemistry and Biochemistry, School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Small Methods. 2020 Sep 11;4(9). doi: 10.1002/smtd.202000125. Epub 2020 Jul 1.
It is a major challenge to coordinate topographic cues from scaffolds with the on-demand, sustained release of biological effectors to maximize their performance in tissue regeneration. Here, a system involving masked, photo-triggered release of biological effectors from a temperature-sensitive scaffold for augmented cell migration and neurite outgrowth is reported. The scaffold contains microparticles of a phase-change material (PCM) sandwiched between two layers of electrospun fibers. The biological effectors are co-loaded with a photothermal dye in the PCM microparticles. Under irradiation with a near-infrared laser, the PCM will be melted to swiftly release the biological effectors. By imposing a photomask between the scaffold and the laser, only those microparticles in the irradiated region are melted, enabling a spatial control over the release. By adjusting the photomask, different regions of the scaffold can be sequentially irradiated at designated times, realizing on-demand and sustained release of the biological effectors with spatiotemporal controls. In one demonstration, this method is used to accelerate the directional migration of NIH-3T3 fibroblasts along the uniaxial or radial direction of fiber alignment by controlling the release of epidermal growth factor. In another demonstration, the release of nerve growth factor is managed to significantly promote neurite outgrowth from PC12 cells.
协调来自支架的地形线索与生物效应物的按需、持续释放，以最大限度地提高它们在组织再生中的性能，是一项重大挑战。在此，报道了一种系统，该系统涉及从温度敏感支架中通过光触发释放生物效应物，以增强细胞迁移和神经突生长。该支架包含夹在两层电纺纤维之间的相变材料（PCM）微粒。生物效应物与光热染料共同负载在PCM微粒中。在近红外激光照射下，PCM将熔化以迅速释放生物效应物。通过在支架和激光之间施加光掩膜，只有照射区域的那些微粒会熔化，从而实现对释放的空间控制。通过调整光掩膜，可以在指定时间依次照射支架的不同区域，实现生物效应物的时空控制下的按需和持续释放。在一个演示中，该方法用于通过控制表皮生长因子的释放来加速NIH-3T3成纤维细胞沿纤维排列的单轴或径向方向的定向迁移。在另一个演示中，通过控制神经生长因子的释放，显著促进了PC12细胞的神经突生长。
