Sun Wan, Tang Fang, Cui Jing-Xue, Lu Zhong-Lin
Shandong Provincial Engineering Laboratory of Novel Pharmaceutical Excipients, Sustained and Controlled Release Preparations, College of Medicine and Nursing, Dezhou University, Dezhou 253023, China.
Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
ACS Omega. 2020 Dec 1;5(49):31700-31705. doi: 10.1021/acsomega.0c04213. eCollection 2020 Dec 15.
Fluorescence detection is desirable to track the gene transfer process in order to explain the mechanism. Here, a fluorescent nanoparticle, diketopyrrolopyrrole-based liposome (), was prepared for DNA delivery and tumor imaging and . The process to deliver DNA into cells was detected in real time by according to the fluorescent property. The transfection efficacies (TEs) for luciferase and enhanced green fluorescent protein (EGFP) analysis of were 1.5 times those of the commercial transfection agent . Importantly, the /DNA system has high EGFP TE with tumor targeting ability. This work provided an effective strategy for monitoring transfection processes.
为了解释基因转移过程的机制，荧光检测对于追踪该过程是很有必要的。在此，制备了一种基于二酮吡咯并吡咯的荧光纳米颗粒脂质体（）用于DNA递送和肿瘤成像。根据荧光特性，通过实时检测将DNA递送至细胞的过程。对荧光素酶和增强型绿色荧光蛋白（EGFP）分析的转染效率（TEs）是市售转染试剂的1.5倍。重要的是，/DNA系统具有高EGFP TE并具有肿瘤靶向能力。这项工作为监测转染过程提供了一种有效的策略。
