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紫外线胁迫对营养生长期不同生长时期青稞草功能性植物化学物质影响的综合评价

Comprehensive Evaluation of the Effect of Ultraviolet Stress on Functional Phytochemicals of Hulless Barley (Qingke) Grass in Different Growth Times at Vegetative Stage.

作者信息

Jiang Bianling, Geng Fuyun, Chang Ran, Ruan Mengting, Bian Ying, Xu Lisheng, Feng Fan, Chen Qiong

机构信息

School of Biological and Food Engineering, Suzhou University, No. 209 Room, Shiyan Building, No. 49, Bianhezhong Road, Yongqiao District, Suzhou 234000, Anhui Province, China.

出版信息

ACS Omega. 2020 Dec 1;5(49):31810-31820. doi: 10.1021/acsomega.0c04576. eCollection 2020 Dec 15.

Abstract

The present study was executed to analyze the functional phytochemicals of hulless barley grass grown under different intensities of ultraviolet stress. The wheat seedlings were imposed to 0.5, 1.0, 1.5, 2.0, and 2.5 h ultraviolet radiation and harvested in different times at vegetative stage. Specifically, the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, and chlorophyll were determined and antioxidants capacity was evaluated by OH and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging ability. A mathematical model (Technique for Order Preference by Similarity to Ideal Solution, TOPSIS) was also employed for the comprehensive evaluation of functional components of hulless barley grass at different growth stages. The results showed that the UV stress could efficiently improve/preserve the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, chlorophyll , chlorophyll , and total chlorophyll, as well as the OH and ABTS scavenging capacity. TOPSIS evaluation revealed that the highest phytochemical contents were yield on the 15th day under 1.0 h ultraviolet treatment.

摘要

本研究旨在分析不同强度紫外线胁迫下种植的青稞草的功能性植物化学物质。将小麦幼苗分别进行0.5、1.0、1.5、2.0和2.5小时的紫外线辐射处理,并在营养生长阶段的不同时间进行收获。具体测定了总多酚、总黄酮、总三萜、总多糖、原花青素和叶绿素的含量,并通过羟基自由基(OH)清除能力和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)(ABTS)清除能力评估抗氧化能力。还采用一种数学模型(逼近理想解排序法,TOPSIS)对不同生长阶段青稞草的功能成分进行综合评价。结果表明,紫外线胁迫可有效提高/保持总多酚、总黄酮、总三萜、总多糖、原花青素、叶绿素a、叶绿素b和总叶绿素的含量,以及羟基自由基清除能力和ABTS清除能力。TOPSIS评价显示,在1.0小时紫外线处理下,第15天的植物化学物质含量最高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee58/7745435/126863a5be10/ao0c04576_0002.jpg

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