Department of Food Science and Biotechnology, Gachon University, Seongnam, Republic of Korea.
J Sci Food Agric. 2022 Mar 30;102(5):1995-2002. doi: 10.1002/jsfa.11538. Epub 2021 Oct 5.
Wheatgrass and barley grass are freshly sprouted leaves of wheat and barley seeds and are rich sources of phytochemicals. This study was conducted to investigate the effects of drought stress on the biochemical compounds and antioxidant activities of barley grass and wheatgrass extracts. The grass was cultivated in an organic soil growing medium with different levels of drought stress (a control with 100% water holding capacity (WHC), mild drought stress with 75% WHC, moderate drought stress with 50% WHC, and severe drought stress with 25% WHC) in a growth chamber by controlling temperature (20/15 °C, day/night), light (12/12 h, light/dark; intensity 150 μmol m s with quantum dot light-emitting diodes), and relative humidity (60%) for 7 days. The drought stress showed increased levels of biochemical compounds, especially phenolics, flavonoids, and vitamin C, in both barley grass and wheatgrass extracts. The wheatgrass extracts showed 1.38-1.67 times higher phenolics, flavonoids, and vitamin C contents than the barley grass extracts did. The antioxidant (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, and nitrite-scavenging activity) and antioxidant enzymes (guaiacol peroxidase, catalase, and glutathione reductase) were the highest under severe drought stress in both barley grass and wheatgrass extracts; and the wheatgrass extracts showed 1.20-5.70 times higher antioxidant enzyme activities than the barley grass extracts did. Proper drought-stress treatment of barley grass and wheatgrass may be a convenient and efficient method to increase biochemical compounds and antioxidants in our diet to exploit the related health benefits. © 2021 Society of Chemical Industry.
小麦草和大麦草是小麦和大麦种子的新鲜嫩芽，是植物化学物质的丰富来源。本研究旨在调查干旱胁迫对大麦草和小麦草提取物生化化合物和抗氧化活性的影响。在生长室中，通过控制温度（20/15°C，白天/黑夜）、光照（12/12 h，光照/黑暗；强度为 150 μmol·m-2·s-1 的量子点发光二极管）和相对湿度（60%），在有机土壤生长介质中用不同水平的干旱胁迫（持水能力 100%（WHC）的对照、75%WHC 的轻度干旱胁迫、50%WHC 的中度干旱胁迫和 25%WHC 的重度干旱胁迫）来种植草。干旱胁迫导致大麦草和小麦草提取物中的生化化合物水平升高，特别是酚类、类黄酮和维生素 C。小麦草提取物中的酚类、类黄酮和维生素 C 含量比大麦草提取物分别高出 1.38-1.67 倍。在大麦草和小麦草提取物中，抗氧化剂（2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）、2,2-二苯基-1-苦基肼自由基清除活性和亚硝酸盐清除活性）和抗氧化酶（愈创木酚过氧化物酶、过氧化氢酶和谷胱甘肽还原酶）在重度干旱胁迫下最高；小麦草提取物中的抗氧化酶活性比大麦草提取物分别高出 1.20-5.70 倍。适当的干旱胁迫处理大麦草和小麦草可能是一种方便有效的方法，可以增加饮食中的生化化合物和抗氧化剂，以利用相关的健康益处。 © 2021 英国化学学会。
