Klimek Robin, Wang Mantian, McKenney Vivien R, Schuman Erin M, Heckel Alexander
Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Max-von-Laue Str. 9, 60438 Frankfurt a.M, Germany.
Max Planck Institute for Brain Research, Max-von-Laue-Str. 4, 60438 Frankfurt a.M, Germany.
Chem Commun (Camb). 2021 Jan 21;57(5):615-618. doi: 10.1039/d0cc06704k.
We developed a superior class of light-activatable molecular beacons with photo-tethered loop regions. Two simple modifications and probe cyclisation prevent the molecular beacon from hybridising with the target RNA before light-activation. Full activity of the molecular beacon is elicited upon illumination with 365 nm light.
我们开发了一类具有光束缚环区域的高级光激活分子信标。两个简单的修饰和探针环化可防止分子信标在光激活前与目标RNA杂交。用365 nm光照射可引发分子信标的全部活性。
