Goethe University Frankfurt, Frankfurt Institute for Molecular Life Sciences (FMLS), Max-von-Laue-Str. 15, 60438 Frankfurt (M), Germany.
Chem Commun (Camb). 2012 Mar 14;48(22):2746-8. doi: 10.1039/c2cc16654b. Epub 2011 Dec 7.
We have synthesized molecular beacons with caged nucleobases in the loop region. These constructs remain non-fluorescent in the presence of their target RNA but can be fully activated by light (366 or 405 nm) in vitro and in HEK293 cells. We suggest that this technology will be useful for single molecule RNA tracking in living cells.
我们合成了带有环区笼状碱基的分子信标。这些结构在存在靶 RNA 时是非荧光的，但可以在体外和 HEK293 细胞中被光（366 或 405nm）完全激活。我们认为这项技术将有助于在活细胞中进行单分子 RNA 追踪。
