Sugiyama Takeshi, Hobro Alison J, Pavillon Nicolas, Umakoshi Takayuki, Verma Prabhat, Smith Nicholas
Department of Applied Physics, Graduate School of Engineering, Osaka University, Japan.
Analyst. 2021 Feb 21;146(4):1268-1280. doi: 10.1039/d0an02077j. Epub 2020 Dec 21.
Macrophage uptake and metabolism of fatty acids is involved in a large number of important biological pathways including immune activation and regulation of macrophages, as well as pathological conditions including obesity, atherosclerosis, and others lifestyle diseases. There are few methods available to directly probe both the uptake and later redistribution/metabolism of fatty acids within living cells as well as the potential changes induced within the cells themselves. We use Raman imaging and analysis to evaluate the effects of different fatty acids following their uptake in macrophages. The label-free nature of the methods means that we can evaluate the fatty acid dynamics without modifying endogenous cellular behavior and metabolism.
巨噬细胞对脂肪酸的摄取和代谢涉及大量重要的生物学途径，包括巨噬细胞的免疫激活和调节，以及肥胖、动脉粥样硬化等病理状况和其他生活方式疾病。目前几乎没有方法可直接探测活细胞内脂肪酸的摄取及其随后的重新分布/代谢，以及细胞自身内部引发的潜在变化。我们使用拉曼成像和分析来评估不同脂肪酸被巨噬细胞摄取后的影响。该方法的无标记特性意味着我们可以在不改变内源性细胞行为和代谢的情况下评估脂肪酸动态。
