Maev I V, Andreev D N, Govorun V M, Ilina E N, Kucheryavyy Y A, Oganesian T S, Melnikova E V, Zayratyants O V, Parfenova T V, Dzhedzheia L V, Kirillova N V, Maevskaya E A, Fomenko A K, Lobanova E G, Zaborovskii A V, Kriukov K A
Yevdokimov Moscow State University of Medicine and Dentistry.
Federal Research And Clinical Center of Physical-Chemical Medicine Federal Medical Biological Agency.
Ter Arkh. 2020 Sep 3;92(8):24-28. doi: 10.26442/00403660.2020.08.000761.
Determine the primary antibiotic resistance of Helicobacter pylori (H. pylori) strains isolated from patients living in the European part of the Russian Federation.
As part of a clinical laboratory study, from 2015 to 2018, 27 gastrobiopsy samples obtained from H. pylori-infected patients were analyzed. H. pylori infection was verified using a rapid urease test or a 13C-urea breath test. The values of the minimum inhibitory concentration (MIC) of antibiotics were determined by the diffusion method using E-test strips (BioMerieux, France) according to the recommendations of the manufacturer. The sensitivity of the isolates was determined for 6 antibacterial drugs (amoxicillin, clarithromycin, metronidazole, levofloxacin, tetracycline, rifampicin).
According to the data obtained, resistance to amoxicillin was 0%, clarithromycin 11.1%, metronidazole 59.3%, levofloxacin 3.7%, tetracycline 0%, and rifampicin 14.8%. Dual resistance to clarithromycin and metronidazole was recorded in two isolates (7.4%).
Thus, the first results of the evaluation of H. pylori antibiotic resistance in the European part of the Russian Federation indicate a low resistance of the microorganism to clarithromycin and quite high to metronidazole.
确定从俄罗斯联邦欧洲部分居民中分离出的幽门螺杆菌（H. pylori）菌株的主要抗生素耐药性。
作为一项临床实验室研究的一部分，在2015年至2018年期间，对从幽门螺杆菌感染患者身上获取的27份胃活检样本进行了分析。使用快速尿素酶试验或13C尿素呼气试验来验证幽门螺杆菌感染。根据制造商的建议，使用E-test试纸条（法国生物梅里埃公司）通过扩散法测定抗生素的最低抑菌浓度（MIC）值。测定了分离株对6种抗菌药物（阿莫西林、克拉霉素、甲硝唑、左氧氟沙星、四环素、利福平）的敏感性。
根据获得的数据，对阿莫西林的耐药率为0%，克拉霉素为11.1%，甲硝唑为59.3%，左氧氟沙星为3.7%，四环素为0%，利福平为14.8%。在两个分离株中记录到对克拉霉素和甲硝唑的双重耐药（7.4%）。
因此，俄罗斯联邦欧洲部分幽门螺杆菌抗生素耐药性评估的初步结果表明，该微生物对克拉霉素的耐药性较低，而对甲硝唑的耐药性相当高。
