Ji Wenliang, Liu Kun, Zhao Gang, Wu Fei, Jiang Ying, Hou Lijuan, Zhang Meining, Mao Lanqun
Department of Chemistry, Renmin University of China, Beijing 100872, China.
Capital University of Physical Education and Sports, Beijing 100191, China.
ACS Sens. 2021 Feb 26;6(2):546-552. doi: 10.1021/acssensors.0c02326. Epub 2020 Dec 21.
Physical exercise (PE) has been drawing increasing attention to prevent and alleviate neural damage of brain diseases; however, in vivo sensing of the neuroprotection ability of PE remains a challenge. Here, we find that ascorbate can be used as a small molecular index for neuroprotective function of PE and the neuroprotection ability of PE can thus be in vivo monitored with an online electrochemical system (OECS) in freely moving animals. With the OECS as the sensing system, we find that the concentration of ascorbate in the microdialysate from the striatum increases greatly in kainic acid (KA)-induced seizure rats and reaches twice the basal level (i.e., 214.4 ± 32.7%, < 0.001, = 4) at a time point 90 min after KA microinjection. Such an increase of ascorbate is obviously attenuated (i.e., 153.6 ± 23.9% of the basal level, < 0.05, = 3) after PE, showing the neuroprotective activity of PE. This finding is believed to be significant in providing chemical insight into the neuroprotection ability of PE.
体育锻炼（PE）在预防和减轻脑部疾病的神经损伤方面越来越受到关注；然而，对体育锻炼神经保护能力的体内传感仍然是一项挑战。在这里，我们发现抗坏血酸可以用作体育锻炼神经保护功能的小分子指标，因此体育锻炼的神经保护能力可以在自由活动的动物中通过在线电化学系统（OECS）进行体内监测。以OECS作为传感系统，我们发现在 kainic 酸（KA）诱导的癫痫大鼠中，纹状体微透析液中抗坏血酸的浓度大幅增加，在KA微量注射后90分钟的时间点达到基础水平的两倍（即214.4±32.7%，<0.001，=4）。体育锻炼后，抗坏血酸的这种增加明显减弱（即基础水平的153.6±23.9%，<0.05，=3），显示了体育锻炼的神经保护活性。这一发现被认为在为体育锻炼的神经保护能力提供化学见解方面具有重要意义。
