Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; College of Agriculture and Animal Husbandry, Tibet University, Nyingchi 860000, China.
Fungal Genet Biol. 2021 Feb;147:103505. doi: 10.1016/j.fgb.2020.103505. Epub 2020 Dec 30.
Cordyceps guangdongensis is a well-known fungus with high nutritional and medicinal value. The metabolite profile of C. guangdongensis is similar to that of Ophiocordyceps sinensis. In plants and animals, microRNAs play important roles in regulating gene expression at the post-transcriptional level. MicroRNA-like RNAs (milRNAs) have been documented in several macro-fungi. To comprehensively investigate the milRNAs in C. guangdongensis, three small RNA libraries from the differentially developmental stages were constructed. Twenty-six conserved milRNAs were identified, and 19 novel milRNA candidates were predicted. Among them, 20 milRNAs were differentially expressed across the developmental processes, and 12 milRNAs were verified using stem-loop quantitative real-time reverse transcription polymerase chain reaction. In addition, the potential target genes of milRNA were predicted to be involved in the development of fruiting bodies and metabolite biosynthesis. This study is the first to report the milRNAs of C. guangdongensis, and provides important insights into studies of milRNA regulation pathways in ascomycete fungi.
广东虫草是一种具有高营养价值和药用价值的知名真菌。广东虫草的代谢物谱与冬虫夏草相似。在植物和动物中，microRNAs 在转录后水平上对基因表达起着重要的调控作用。在几种大型真菌中已经记录了 microRNA 样 RNA (milRNAs)。为了全面研究广东虫草中的 milRNAs，我们构建了三个来自不同发育阶段的小 RNA 文库。鉴定出 26 个保守的 milRNAs，并预测了 19 个新的 milRNA 候选物。其中，20 个 milRNAs 在发育过程中差异表达，12 个 milRNAs 通过茎环定量实时逆转录聚合酶链反应得到验证。此外，还预测了 milRNA 的潜在靶基因参与了子实体的发育和代谢产物的生物合成。本研究首次报道了广东虫草的 milRNAs，为研究子囊菌真菌中 milRNA 调控途径提供了重要的见解。
