Rosero-Salazar Doris H, Carvajal-Monroy Paola L, Wagener Frank A D T G, Von den Hoff Johannes W
Department of Orthodontics and Craniofacial Biology, Radboud University Medical Centre, Nijmegen, the Netherlands; Department of Medical Basic Sciences, Faculty of Health, Universidad Icesi, Cali, Colombia.
Department of Orthodontics and Craniofacial Biology, Radboud University Medical Centre, Nijmegen, the Netherlands; Department of Oral and Maxillofacial Surgery, Special Dental Care and Orthodontics, Erasmus Medical Center, Rotterdam, the Netherlands.
Arch Oral Biol. 2021 Feb;122:105021. doi: 10.1016/j.archoralbio.2020.105021. Epub 2020 Dec 8.
The aim of this study was to analyze the function of the palatal muscles in vivo by real-time wireless electromyography in rats. The effects of palatal wounding were also analyzed.
Microelectrodes were implanted six rats; in the masseter muscle (two-rats) for comparison, in the unwounded soft palate (two-rats) and the soft palate that received a surgical wound (two-rats). Two weeks after implantation, a wound was made in the soft palate using a 1 mm biopsy-punch. Electromyographic measurements and video-recordings were taken weekly to monitor train-duration and peak-amplitude during eating, grooming and drinking.
The train-duration of the masseter muscle during eating was 0.49 ± 0.11 s (rat-1) and 0.56 ± 0.09 s (rat-2), which was higher than during grooming. In the unwounded soft palate the train-duration during eating was 0.63 ± 0.12 s (rat-1) and 0.69 ± 0.069 s (rat-2), which was higher than during grooming and drinking. The peak-amplitude for eating in the normal soft palate before surgery was 0.31 ± 0.001 mV (rat-1) and 0.33 ± 0.02 mV (rat-2). This decreased to 0.23 ± 0.03 mV and 0.25 ± 0.11 mV respectively, after surgery. For drinking the peak-amplitude was 0.30 ± 0.01 mV (rat-1) and 0.39 ± 0.01 mV (rat-2) before surgery, which decreased to 0.23 ± 0.09 mV and 0.20 ± 0.14 mV respectively, after surgery.
The reduced peak-amplitude suggests impaired soft palate function after wounding. This is the first study into the in vivo function of the soft palate after surgical wounding. This model will contribute to develop strategies to improve soft palate function in patients.
本研究旨在通过大鼠实时无线肌电图分析腭肌在体内的功能。同时分析腭部损伤的影响。
将微电极植入6只大鼠体内；2只大鼠植入咬肌用于对比，2只大鼠植入未受伤的软腭，2只大鼠植入接受手术创伤的软腭。植入两周后，用1毫米活检打孔器在软腭上造成伤口。每周进行肌电图测量和视频记录，以监测进食、梳理毛发和饮水时的动作持续时间和峰值幅度。
进食时咬肌的动作持续时间，大鼠1为0.49±0.11秒，大鼠2为0.56±0.09秒，高于梳理毛发时。在未受伤的软腭中，进食时的动作持续时间，大鼠1为0.63±0.12秒，大鼠2为0.69±0.069秒，高于梳理毛发和饮水时。手术前正常软腭进食时的峰值幅度，大鼠1为0.31±0.001毫伏，大鼠2为0.33±0.02毫伏。手术后分别降至0.23±0.03毫伏和0.25±0.11毫伏。饮水时的峰值幅度，手术前大鼠1为0.30±0.01毫伏，大鼠2为0.39±0.01毫伏，手术后分别降至0.23±0.09毫伏和0.20±0.14毫伏。
峰值幅度降低表明受伤后软腭功能受损。这是第一项关于手术创伤后软腭体内功能的研究。该模型将有助于制定改善患者软腭功能的策略。
