Department of Dentistry, Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre, 6525EX Nijmegen, The Netherlands.
Department of Medical Basic Sciences, Faculty of Health, Universidad Icesi, Cali 760008, Colombia.
Biomolecules. 2021 Oct 19;11(10):1547. doi: 10.3390/biom11101547.
This study aimed to analyze the effects of fibrin constructs enhanced with laminin-nidogen, implanted in the wounded rat soft palate. Fibrin constructs with and without laminin-nidogen were implanted in 1 mm excisional wounds in the soft palate of 9-week-old rats and compared with the wounded soft palate without implantation. Collagen deposition and myofiber formation were analyzed at days 3, 7, 28 and 56 after wounding by histochemistry. In addition, immune staining was performed for a-smooth muscle actin (a-SMA), myosin heavy chain (MyHC) and paired homeobox protein 7 (Pax7). At day 56, collagen areas were smaller in both implant groups (31.25 ± 7.73% fibrin only and 21.11 ± 6.06% fibrin with laminin-nidogen)) compared to the empty wounds (38.25 ± 8.89%, < 0.05). Moreover, the collagen area in the fibrin with laminin-nidogen group was smaller than in the fibrin only group ( ˂ 0.05). The areas of myofiber formation in the fibrin only group (31.77 ± 10.81%) and fibrin with laminin-nidogen group (43.13 ± 10.39%) were larger than in the empty wounds (28.10 ± 11.68%, ˂ 0.05). Fibrin-based constructs with laminin-nidogen reduce fibrosis and improve muscle regeneration in the wounded soft palate. This is a promising strategy to enhance cleft soft palate repair and other severe muscle injuries.
本研究旨在分析在创伤性大鼠软腭中植入纤维蛋白构建体增强层粘连蛋白-巢蛋白的效果。将含有和不含有层粘连蛋白-巢蛋白的纤维蛋白构建体植入 9 周龄大鼠软腭 1mm 切除性创伤中，并与未植入创伤的软腭进行比较。通过组织化学分析在创伤后第 3、7、28 和 56 天分析胶原蛋白沉积和肌纤维形成。此外，还进行了α-平滑肌肌动蛋白（α-SMA）、肌球蛋白重链（MyHC）和配对同源框蛋白 7（Pax7）的免疫染色。在第 56 天，与空伤组（38.25 ± 8.89%）相比，两个植入组（纤维蛋白仅 31.25 ± 7.73%，纤维蛋白+层粘连蛋白-巢蛋白 21.11 ± 6.06%）的胶原蛋白面积较小（ ˂ 0.05）。此外，纤维蛋白+层粘连蛋白-巢蛋白组的胶原蛋白面积小于纤维蛋白组（ ˂ 0.05）。纤维蛋白组（31.77 ± 10.81%）和纤维蛋白+层粘连蛋白-巢蛋白组（43.13 ± 10.39%）的肌纤维形成面积大于空伤组（28.10 ± 11.68%， ˂ 0.05）。含有层粘连蛋白-巢蛋白的纤维蛋白基构建体可减少创伤性软腭中的纤维化并改善肌肉再生。这是增强腭裂软腭修复和其他严重肌肉损伤的有前途的策略。
