Ichihara K, Takahashi T, Fujii S
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
Biochim Biophys Acta. 1988 Jan 19;958(1):125-9. doi: 10.1016/0005-2760(88)90253-6.
Diacylglycerol acyltransferase in a particulate preparation from maturing safflower seeds showed no strict selectivity for acyl-CoA when acyl-CoA substrates were administered as mixtures. This suggested that the fatty acid composition of position 3 in safflower triacyl-sn-glycerol exclusively depends on the acyl-CoA composition in the cell. The specific activity of the acylation was approximately 3 nmol/min per mg protein of the preparation under the optimum assay conditions. This low activity and other data appeared to indicate that the diacylglycerol acyltransferase reaction may be the rate-limiting step in triacylglycerol synthesis in vivo.
当以混合物形式提供酰基辅酶A底物时，来自成熟红花种子的微粒体制剂中的二酰甘油酰基转移酶对酰基辅酶A没有严格的选择性。这表明红花三酰基-sn-甘油中3位的脂肪酸组成仅取决于细胞中的酰基辅酶A组成。在最佳测定条件下，该酰化反应的比活性约为每毫克制剂蛋白3 nmol/分钟。这种低活性和其他数据似乎表明，二酰甘油酰基转移酶反应可能是体内三酰甘油合成中的限速步骤。
