北温带人工林与原始红松林凋落物层微生物群落和代谢产物的差异
Differences in Microbial Community and Metabolites in Litter Layer of Plantation and Original Korean Pine Forests in North Temperate Zone.
作者信息
Wang Yue, Li Ting, Li Chongwei, Song Fuqiang
机构信息
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China.
Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization, College of Life Science, Heilongjiang University, Harbin 150080, China.
出版信息
Microorganisms. 2020 Dec 17;8(12):2023. doi: 10.3390/microorganisms8122023.
In order to explore the relationship between microbial diversity and metabolites in the litter layer of northern temperate forests, the microbial community structure and metabolite species in the litter layer of an original Korean pine forest and Korean pine plantation of northern temperate climate were determined on the basis of high-throughput sequencing and metabonomic techniques. The results showed that there were 698 bacterial genera and 363 fungal genera in the litter samples in the original Korean pine forest. Linear discriminant effect size (LEfSe) analysis showed that there were 35 indicator bacterial species and 19 indicator fungal species. In the litter samples of the Korean pine plantation, there were 622 bacterial genera and 343 fungal genera. Additionally, LEfSe analysis showed that there were 18 indicator bacterial species and 5 indicator fungal species. The litter of the two forest types contained 285 kinds of organic compounds, among which 16 different metabolites were screened, including 6 kinds of organic acids, 5 kinds of amino acids, 2 kinds of sugars, 2 kinds of sugar alcohols, and 1 kind of lipid. Latescibacteria, Rokubacteria, and Olpidiomycota are unique to the original Korean pine forest. They can catalyze the degradation rate of litter and decompose cellulose and chitin, respectively. Subgroup 6 was abundant in the lower litter layer. Subgroup 6 can grow with carbon compounds as substrate. It was clear that the microbial diversity of the litter layer in the original Korean pine forest was higher than that of the Korean pine plantation. Moreover, whether original forest or plantation forest, the lower-litter layer microbial diversity was higher than that in the middle-litter layer. CCA showed that the main metabolites were related to were saccharopine. The main metabolites associated with and were myo-inositol. At the same time, analysis of the difference between the litter layer of the original Korean pine forest and the Korean pine plantation also provides a theoretical basis for their participation in the element cycles of forest ecosystems.
为了探究北温带森林凋落物层中微生物多样性与代谢产物之间的关系，基于高通量测序和代谢组学技术，测定了北温带气候下原始红松林和红松人工林凋落物层的微生物群落结构和代谢产物种类。结果表明，原始红松林凋落物样本中有698个细菌属和363个真菌属。线性判别效应大小（LEfSe）分析表明，有35种指示细菌物种和19种指示真菌物种。在红松人工林凋落物样本中，有622个细菌属和343个真菌属。此外，LEfSe分析表明，有18种指示细菌物种和5种指示真菌物种。两种森林类型的凋落物含有285种有机化合物，其中筛选出16种不同的代谢产物，包括6种有机酸、5种氨基酸、2种糖类、2种糖醇类和1种脂质。缓生根瘤菌、罗库菌和油壶菌门是原始红松林特有的。它们可以分别催化凋落物的降解速率并分解纤维素和几丁质。第6亚群在凋落物下层含量丰富。第6亚群可以以碳化合物为底物生长。很明显，原始红松林凋落物层的微生物多样性高于红松人工林。此外，无论是原始林还是人工林，凋落物下层的微生物多样性都高于中层。冗余分析（CCA）表明，主要代谢产物与saccharopine有关。与[具体内容缺失]和[具体内容缺失]相关的主要代谢产物是肌醇。同时，对原始红松林和红松人工林凋落物层差异的分析也为它们参与森林生态系统的元素循环提供了理论依据。
Zotero 插件