Nyberg F, Lindström L H, Terenius L
Department of Pharmacology, University of Uppsala, Sweden.
Biol Psychiatry. 1988 Jan 15;23(2):115-22. doi: 10.1016/0006-3223(88)90080-7.
Defatted breast milk from women with postpartum psychosis and from healthy lactating women was analyzed by high-resolution gel permeation chromatography as well as by sodium dodecyl sulfate (SDS) and urea polyacrylamide gel electrophoresis. The gel permeation procedure allowed quantitative analysis of milk proteins (including beta-casein) with minute amounts of defatted milk (10-15 microliter). By electrophoresis, further characterization of the protein pattern, including the beta-casein fraction, was obtained. Milk samples from five control and seven psychotic subjects were analyzed. The concentration of the beta-casein-containing peak was significantly lower in milk samples from the psychotic group by both chromatography and electrophoresis. These lower levels of beta-casein may result from a higher rate of enzymatic degradation generating i.a. peptides with opioid activity, as shown earlier in plasma and CSF of women with postpartum psychosis.
采用高分辨率凝胶渗透色谱法以及十二烷基硫酸钠（SDS）和尿素聚丙烯酰胺凝胶电泳法，对产后精神病患者和健康哺乳期妇女的脱脂母乳进行了分析。凝胶渗透程序可对微量脱脂乳（10 - 15微升）中的乳蛋白（包括β-酪蛋白）进行定量分析。通过电泳，可进一步表征蛋白质图谱，包括β-酪蛋白组分。对来自5名对照受试者和7名精神病受试者的乳汁样本进行了分析。通过色谱法和电泳法均发现，精神病组乳汁样本中含β-酪蛋白的峰浓度显著较低。这些较低水平的β-酪蛋白可能是由于酶促降解速率较高，从而产生了具有阿片样活性的肽，正如之前在产后精神病患者的血浆和脑脊液中所显示的那样。
