Renlund S, Erlandsson I, Hellman U, Silberring J, Wernstedt C, Lindström L, Nyberg F
Pharmacia LKB Biotechnology AB, Uppsala, Sweden.
Peptides. 1993 Nov-Dec;14(6):1125-32. doi: 10.1016/0196-9781(93)90165-d.
Milk was obtained from a woman with acute postpartum psychosis and with ongoing lactation. Defatted samples were subjected to micropurification and collected fractions were analyzed by means of their beta-casomorphin-8 immunoreactivity. Immunoreactive material with the same chromatographic properties as synthetic human beta-casomorphin-8 was determined by amino acid sequence analysis to be Tyr-Pro-Phe-Val-Glu-Pro-Ile-Pro. Its molecular mass was determined by fast atom bombardment-mass spectrometry to be 962.3 Da. These determinations, which ultimately identify the immunoreactive material as human beta-casomorphin-8, represent the first structural identification of a beta-casomorphin peptide from a body fluid.
乳汁取自一名患有急性产后精神病且仍在泌乳的女性。脱脂样本经过微纯化处理，收集的馏分通过其β-酪蛋白吗啡-8免疫反应性进行分析。通过氨基酸序列分析确定，具有与合成人β-酪蛋白吗啡-8相同色谱特性的免疫反应性物质为Tyr-Pro-Phe-Val-Glu-Pro-Ile-Pro。通过快原子轰击质谱法测定其分子量为962.3道尔顿。这些最终将免疫反应性物质鉴定为人β-酪蛋白吗啡-8的测定结果，代表了首次从体液中对β-酪蛋白吗啡肽进行结构鉴定。
