Norioun Hamid, Motovali-Bashi Majid, Javadirad Seyed Morteza
Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Int J Reprod Biomed. 2020 Nov 22;18(11):961-968. doi: 10.18502/ijrm.v13i11.7963. eCollection 2020 Nov.
The role of and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility.
The aim of this study was to evaluate the expression level of in azoospermic men to reveal its possible association with infertility.
This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RT-qPCR) method was used and statistical analysis was performed to compare the expression level of in both OA and NOA individuals.
In silico analysis suggested , with its potential binding ability to target transcripts. The expression analysis of candidate indicated its significant overexpression in NOA men.
The was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly and indirectly and . Therefore, spermatogenesis could be misled and male infertility could be developed.
在动物模型中，[基因名称]及其下游基因在男性生育中的作用已得到证实。此外，[基因名称]的表达下调与人类无精子症表型显著相关。微小RNA的异常表达可能误导精子发生，并大多导致男性不育的多种表型。
本研究旨在评估无精子症男性中[基因名称]的表达水平，以揭示其与不育的可能关联。
本病例对照研究对30例无精子症男性进行，根据病理检查，其中19例为非梗阻性无精子症（NOA），11例为梗阻性无精子症（OA）。安全地进行了全面的生物信息学研究，随后选择了[基因名称]。采用逆转录定量聚合酶链反应（RT-qPCR）方法，并进行统计分析以比较OA和NOA个体中[基因名称]的表达水平。
计算机分析表明[基因名称]具有靶向[转录本名称]转录本的潜在结合能力。候选[基因名称]的表达分析表明其在NOA男性中显著过表达。
与OA对照个体相比，[基因名称]在NOA男性中过表达。因此，过表达的微小RNA可直接下调[基因名称]，并间接下调[基因名称1]和[基因名称2]。因此，精子发生可能被误导并导致男性不育。
