诱导多能干细胞的感应式共培养分化为心肌细胞。
Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes.
机构信息
University of British Columbia, Vancouver, BC, Canada.
Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada.
出版信息
Methods Mol Biol. 2022;2454:117-126. doi: 10.1007/7651_2020_335.
Various types of stem cells and nonstem cells have been shown to differentiate or transdifferentiate into cardiomyocytes by way of coculture with appropriate inducer cells. Here we describe a method to induce cardiac differentiation in induced pluripotent stem (iPS) cells through the use of coculture with previously differentiated iPS cell-derived cardiomyocytes (iCMs). This differentiation process can be achieved without the use of exogenous pathway inhibitors and morphogens.
已经证明，各种类型的干细胞和非干细胞通过与适当的诱导细胞共培养可以分化或转分化为心肌细胞。在这里，我们描述了一种通过与先前分化的诱导多能干细胞（iPS 细胞）衍生的心肌细胞（iCMs）共培养来诱导 iPS 细胞向心肌细胞分化的方法。这个分化过程可以在不使用外源途径抑制剂和形态发生素的情况下实现。
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