School of Bioengineering, Qilu University of Technology, Jinan, China.
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Jinan, China.
J Sci Food Agric. 2021 Aug 15;101(10):4050-4058. doi: 10.1002/jsfa.11040. Epub 2021 Jan 12.
Cassava is rich in nutrition and has high edible value, but the development of the cassava industry is limited by the traditional low added value processing and utilization mode. In this study, cassava tuber was used as beer adjunct to develop a complete set of fermentation technology for manufacturing cassava beer.
The activities of transaminase, phenylpyruvate decarboxylase and dehydrogenase in 2-phenylethanol Ehrlich biosynthesis pathway of Saccharomyces cerevisiae were higher in cassava beer than that of malt beer. Aminotransferase ARO9 gene and phenylpyruvate decarboxylase ARO10 gene were up-regulated in the late stage of fermentation, which indicated that they were the main regulated genes of 2-phenylethanol Ehrlich pathway with phenylalanine as substrate in cassava beer preparation.
Compared with traditional wheat beer, cassava beer was similar in the content of nutrition elements, diacetyl, total acid, alcohol and carbon dioxide, but has the characteristics of fresh fragrance and better taste. The hydrocyanic acid contained in cassava root tubes was catabolized during fermentation and compliant with the safety standard of beverage. Further study found that the content of 2-phenylethanol in cassava beer increased significantly, which gave cassava beer a unique elegant and delicate rose flavor. © 2020 Society of Chemical Industry.
木薯营养丰富，食用价值高，但木薯产业的发展受到传统低附加值加工利用模式的限制。本研究以木薯块根为啤酒辅料，开发了一套完整的木薯啤酒酿造发酵工艺。
与麦芽啤酒相比，木薯啤酒中酿酒酵母苯乙醇 Ehrlich 生物合成途径中转氨酶、苯丙酮酸脱羧酶和脱氢酶的活性更高。在发酵后期，氨基转移酶 ARO9 基因和苯丙酮酸脱羧酶 ARO10 基因上调表达，表明它们是木薯啤酒制备中以苯丙氨酸为底物的苯乙醇 Ehrlich 途径的主要调控基因。
与传统的小麦啤酒相比，木薯啤酒在营养成分、双乙酰、总酸、酒精和二氧化碳含量等方面相似，但具有清香爽口的特点。木薯块根中的氰酸根在发酵过程中被分解，符合饮料的安全标准。进一步的研究发现，木薯啤酒中 2-苯乙醇的含量显著增加，赋予了木薯啤酒独特的优雅细腻的玫瑰风味。 © 2020 英国化学学会。
