利用基因工程改造的马克斯克鲁维酵母从葡萄糖生物合成2-苯乙醇。
Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus.
作者信息
Kim Tae-Yeon, Lee Sang-Woo, Oh Min-Kyu
机构信息
Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
出版信息
Enzyme Microb Technol. 2014 Jul-Aug;61-62:44-7. doi: 10.1016/j.enzmictec.2014.04.011. Epub 2014 Apr 27.
2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG(fbr) from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3g/L of 2-PE was produced from 20g/L glucose without addition of phenylalanine in the medium.
2-苯乙醇（2-PE）是一种具有玫瑰香味的芳香醇，用于化妆品、香料和食品工业。少数酵母菌株通过埃利希途径生产2-PE。在本研究中，对马克斯克鲁维酵母进行基因工程改造，以从葡萄糖过量生产2-PE。通过过表达酿酒酵母的苯丙酮酸脱羧酶（ARO10）和乙醇脱氢酶（ADH2）基因，产生了约1.0g/L的2-PE。从对苯丙氨酸类似物对氟苯丙氨酸具有抗性的进化型马克斯克鲁维酵母中也产生了类似水平的2-PE。来自肺炎克雷伯菌的编码3-脱氧-D-阿拉伯庚糖酸-7-磷酸（DHAP）合酶反馈抗性突变体的aroG（fbr）在进化型马克斯克鲁维酵母中过表达。最后，在培养基中不添加苯丙氨酸的情况下，从20g/L葡萄糖中产生了1.3g/L的2-PE。
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