Chang-Zheng Liu, Liang-Yun Zhou, Pei-Ran Liao, Lei Zhong, Qi-Zhong Cai, Xiao-Lin Jiang, Quan Yang
Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station Chinese Materia Medica Industry Technology System, Key Laboratory for Production & Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2020 Nov;45(22):5452-5458. doi: 10.19540/j.cnki.cjcmm.20200816.103.
To investigate the effect of Polygonum multiflorum-Andrographis paniculata intercropping system on rhizosphere soil actinomycetes of P. multiflorum, the community structure and diversity of soil actinomycetes were studied by using the original soil as the control group and the rhizosphere soil actinomycetes communities of P. multiflorum under monoculture and intercropping systems as the experimental group. In this study 655 221 effective sequences were obtained with an average length of 408 bp. OTU coverage and rarefaction curve showed that the sequencing could represent the real situation of soil actinomycetes. According to the results of alpha diversity analysis, the diversity soil actinomycetes varied as follows: original soil>intercropping soil>monoculture soil. The soil actinomycetes community structure and the relative abundance of dominant genera were significantly changed by both monoculture and intercropping, especially monoculture. OTU clustering and PCA analysis of soil samples showed that all the soil samples were divided into three distinct groups and the original soil was more similar to intercropping soil. In addition, intercropping increased the relative abundance of some beneficial actinomyces, such as Kitasatospora and Mycobacterium, which was beneficial to maintain soil health and reduce the occurrence of soil-borne diseases. The results show that, P. multiflorum-A. paniculata intercropping reduced the change of community structure and the decrease of diversity of soil actinomycetes caused by P. multiflorum monoculture, and made the actinomycete community in rhizosphere soil of P. multiflorum close to the original soil.
为研究何首乌与穿心莲间作系统对何首乌根际土壤放线菌的影响，以原土为对照组，以单作和间作系统下何首乌根际土壤放线菌群落为实验组，研究了土壤放线菌的群落结构和多样性。本研究共获得655 221条有效序列，平均长度为408 bp。OTU覆盖率和稀释曲线表明测序结果能够代表土壤放线菌的实际情况。根据α多样性分析结果，土壤放线菌多样性变化如下：原土＞间作土壤＞单作土壤。单作和间作均显著改变了土壤放线菌群落结构和优势属的相对丰度，尤其是单作。土壤样品的OTU聚类和PCA分析表明，所有土壤样品分为三个不同的组，原土与间作土壤更为相似。此外，间作增加了一些有益放线菌如北里孢菌属和分枝杆菌属的相对丰度，有利于维持土壤健康，减少土传病害的发生。结果表明，何首乌与穿心莲间作减少了何首乌单作引起的土壤放线菌群落结构变化和多样性降低，使何首乌根际土壤放线菌群落接近原土。
