[神经梅毒患者血清及脑脊液中CXCL8、CXCL10及Th1/Th2细胞因子的表达]

[Expressions of CXCL8, CXCL10 and Th1 /Th2 cytokines in the serum and cerebrospinal fluid of patients with neurosyphilis].

作者信息

Yu Xiao-Yun, Yan Ning, Li Zi-Hai, Chen Wei, Hua Yun-Hui, Lu Yan

机构信息

Department of Dermatovenerology, Nanjing Second Hospital / Nanjing Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210003, China.

Department of Dermatovenerology, Jiangsu Provincial People's Hospital / The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

出版信息

Zhonghua Nan Ke Xue. 2020 Apr;26(4):335-340.

PMID:33351301

Abstract

OBJECTIVE

To investigate the levels of chemokines 8 and 10 (CXCL8 and CXCL10), Th1 cytokines (IL-2, IL-12 and IFN-γ) and Th2 cytokines (IL-6 and IL-10) in the serum and cerebrospinal fluid of patients with neurosyphilis and elucidate their roles in the immune response and pathogenesis of neurosyphilis.

METHODS

Using ELISA, we detected the expressions of CXCL8, CXCL10, IL2, IL-2, IFN-γ, IL-6 and IL-10 in the serum and cerebrospinal fluid of 42 cases of neurosyphilis, 44 cases of syphilis and 40 cases of non-inflammatory diseases of the nervous system (the control group).

RESULTS

The serum levels of CXCL8, CXCL10, IL-2, IL-12, IFN-γ, IL-6 and IL-10 were significantly lower in the neurosyphilis group than in the syphilis and control groups (P < 0.05), and so were they in the male than in the female neurosyphilis patients (P < 0.05). However, the expressions of CXCL8, CXCL10, IL-2, IL-12, IFN-γ, IL-6 and IL-10 in the cerebrospinal fluid were remarkably higher in the neurosyphilis group than in the syphilis and control groups (P < 0.05), and so were they in the male than in the female neurosyphilis patients (P < 0.05).

CONCLUSIONS

Patients with neurosyphilis have cellular immune dysfunction, and their immune response involves CXCL8, CXCL10 and Th1 / Th2 cytokines.

摘要

目的

探讨神经梅毒患者血清和脑脊液中趋化因子8和10（CXCL8和CXCL10）、Th1细胞因子（IL-2、IL-12和IFN-γ）及Th2细胞因子（IL-6和IL-10）的水平，阐明它们在神经梅毒免疫反应和发病机制中的作用。

方法

采用酶联免疫吸附测定法（ELISA），检测42例神经梅毒患者、44例梅毒患者及40例神经系统非炎性疾病患者（对照组）血清和脑脊液中CXCL8、CXCL10、IL-2、IL-12、IFN-γ、IL-6和IL-10的表达。

结果

神经梅毒组血清中CXCL8、CXCL10、IL-2、IL-12、IFN-γ、IL-6和IL-10水平显著低于梅毒组和对照组（P<0.05），男性神经梅毒患者低于女性神经梅毒患者（P<0.05）。然而，神经梅毒组脑脊液中CXCL8、CXCL10、IL-2、IL-12、IFN-γ、IL-6和IL-10的表达明显高于梅毒组和对照组（P<0.05），男性神经梅毒患者高于女性神经梅毒患者（P<0.05）。