长链非编码 RNA Gm12840 通过海绵吸附 miR-677-5p 介导 WISP1 调节缺血再灌注诱导的肾纤维化。
LncRNA Gm12840 mediates WISP1 to regulate ischemia-reperfusion-induced renal fibrosis by sponging miR-677-5p.
机构信息
Department of Anesthesiology, Guangzhou Eighth People's Hospital, Guangzhou Medical University, 8 Huaying Road, Guangzhou, Guangdong 510060, PR China.
Department of Anesthesiology, Panyu Central Hospital, 8 Fuyu West Road, Guangzhou, Guangdong 511400, PR China.
出版信息
Epigenomics. 2020 Dec;12(24):2205-2218. doi: 10.2217/epi-2020-0054. Epub 2020 Dec 22.
We aimed to identify that long noncoding RNAs (lncRNAs) are involved in ischemia-reperfusion (IR)-induced late fibrosis of kidney and may constitute novel therapeutic strategies for acute kidney injury-induced chronic kidney disease. We performed the mouse model of IR later induced renal fibrosis and analyzed lncRNA profiles using second-generation sequencing during the pathogenesis. The expression levels of 43 lncRNAs and 141 lncRNAs were respectively changed significantly 7 days and 2 weeks after IR treatment. Based on the correlation analysis of the differentially expressed genes, the interaction networks of lncRNAs, miRNAs and mRNA were structured. LncRNA (Gm12840) could act as a sponge for miR-677-5p to mediate fibroblast activation induced by TGF-β1 via the WISP1/PKB (Akt) signaling pathway.
我们旨在确定长链非编码 RNA（lncRNA）参与缺血再灌注（IR）诱导的肾脏晚期纤维化，并且可能为急性肾损伤诱导的慢性肾脏病构成新的治疗策略。我们进行了 IR 后诱导肾脏纤维化的小鼠模型，并在发病过程中使用第二代测序分析 lncRNA 谱。IR 治疗后 7 天和 2 周，分别有 43 个 lncRNA 和 141 个 lncRNA 的表达水平发生显著变化。基于差异表达基因的相关性分析，构建了 lncRNA、miRNA 和 mRNA 的相互作用网络。lncRNA（Gm12840）可以作为 miR-677-5p 的海绵，通过 WISP1/PKB（Akt）信号通路介导 TGF-β1 诱导的成纤维细胞激活。
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