Department of Gastroenterology, Yantai Affiliated Hospital of Binzhou Medical University, 264100, PR China.
Department of Microbiology, College of Basic Medical Sciences, Binzhou Medical University, 264100, PR China.
Microb Pathog. 2021 Jan;150:104707. doi: 10.1016/j.micpath.2020.104707. Epub 2020 Dec 24.
To investigate the communities of fecal microbiota and the role of Toll-like receptors in patients with ulcerative colitis in the coastal area of northern China.
Stool samples from 31 patients with ulcerative colitis and 12 healthy individuals were collected. The total bacterial genomic DNA was extracted, and the V3+V4 hypervariable region in the bacterial 16S rRNA gene sequence was amplified by polymerase chain reaction (PCR). High-throughput sequencing analysis was performed on the Illumina Hiseq platform. The expression of TLR2, TLR4, Tollip, PPAR-γ, IL-6, and TNF-α in the colonic mucosa was measured by Western blots.
The diversity of the fecal microbiota in patients with ulcerative colitis was significantly less than that in healthy control individuals (p < 0.05). The proportion of Bacteroidetes was significantly reduced (p < 0.01), whereas Proteobacteria was prevalent (p < 0.01) in patients with ulcerative colitis. At the genus level, the relative abundance of Streptococcus and Anaerostipes was significantly increased (p < 0.05), whereas the proportion of Bacteroides, Lachnospira, Ruminococcus, Phascolarctobacterium, and Coprococcus was significantly decreased in patients with ulcerative colitis (p < 0.05). The diversity indexes of fecal microbiota in patients with ulcerative colitis were negatively correlated with disease severity (p < 0.05). The relative abundance of Enterobacteriaceae was positively correlated with disease severity, and the relative abundance of Phascolarctobacterium, Anaerostipes, Fusobacterium, Parabacteroides, Oscillospira, and Ochrobactrum were negatively correlated with disease severity. The expression levels of TLR2 and TLR4 in the intestinal mucosa were positively correlated with the relative abundance of Streptococcus and Enterobacteriaceae, respectively (r = 0.481, p = 0.007; r = 0.455, p = 0.017).
There were significant changes in the diversity and composition of the fecal microbiota in patients with ulcerative colitis compared to healthy individuals. The dysbiosis of gut microbiota and correlation with TLRs might play important roles in the pathogenesis and progression of ulcerative colitis.
研究中国北方沿海地区溃疡性结肠炎患者的粪便微生物群落及其 Toll 样受体的作用。
收集 31 例溃疡性结肠炎患者和 12 例健康对照者的粪便标本。提取总细菌基因组 DNA，采用聚合酶链反应（PCR）扩增细菌 16S rRNA 基因 V3+V4 高变区。在 Illumina Hiseq 平台上进行高通量测序分析。采用 Western blot 法检测结肠黏膜中 TLR2、TLR4、Tollip、PPAR-γ、IL-6 和 TNF-α的表达。
溃疡性结肠炎患者粪便微生物多样性明显低于健康对照组（p<0.05）。拟杆菌门的比例明显降低（p<0.01），而变形菌门在溃疡性结肠炎患者中较为常见（p<0.01）。在属水平上，链球菌和厌氧棒状杆菌的相对丰度明显增加（p<0.05），而拟杆菌属、lachnospira、瘤胃球菌属、phascolarctobacterium 和 coprococcus 的比例在溃疡性结肠炎患者中明显降低（p<0.05）。溃疡性结肠炎患者粪便微生物多样性指数与疾病严重程度呈负相关（p<0.05）。肠杆菌科的相对丰度与疾病严重程度呈正相关，而 phascolarctobacterium、anaerostipes、fusobacterium、parabacteroides、oscillospira 和 ochrobactrum 的相对丰度与疾病严重程度呈负相关。肠黏膜中 TLR2 和 TLR4 的表达水平分别与链球菌和肠杆菌科的相对丰度呈正相关（r=0.481，p=0.007；r=0.455，p=0.017）。
与健康对照者相比，溃疡性结肠炎患者粪便微生物的多样性和组成存在显著变化。肠道微生物群的失调及其与 TLRs 的相关性可能在溃疡性结肠炎的发病机制和进展中发挥重要作用。
