肌原纤维形成对培养的胚胎大鼠肌管中乙酰胆碱酯酶mRNA的影响。

Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes.

作者信息

Younkin L H, McTiernan C F, Younkin S G

机构信息

Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

出版信息

Exp Cell Res. 1988 Jan;174(1):279-81. doi: 10.1016/0014-4827(88)90161-9.

DOI:10.1016/0014-4827(88)90161-9

PMID:3335227

Abstract

Acetylcholinesterase (AChE) and AChE mRNA were evaluated in spontaneously fibrillating myotubes derived from 20-day-old rat fetuses and in matched cultures in which fibrillation was prevented by adding tetrodotoxin on the fourth day of culture. On the eighth day of culture, the AChE activity of fibrillating and nonfibrillating cultures was 5332 and 1861 pmol ACh hydrolyzed min-1 dish-1, respectively (P less than 0.005). Total mRNA was essentially the same in fibrillating and nonfibrillating cultures (27.4 and 25.4 micrograms/dish, respectively). AChE mRNA was assessed by assaying the AChE produced by Xenopus oocytes microinjected with purified mRNA. The AChE produced by mRNA from fibrillating and nonfibrillating cultures was 0.46 and 0.10 pmol ACh hydrolyzed min-1 oocyte-1, respectively (P less than 0.005).

摘要

在源自20日龄大鼠胎儿的自发纤颤肌管以及在培养第4天添加河豚毒素以防止纤颤的配对培养物中，对乙酰胆碱酯酶（AChE）和AChE mRNA进行了评估。在培养的第8天，纤颤培养物和非纤颤培养物的AChE活性分别为5332和1861 pmol乙酰胆碱水解·min⁻¹·培养皿⁻¹（P<0.005）。纤颤培养物和非纤颤培养物中的总mRNA基本相同（分别为27.4和25.4μg/培养皿）。通过测定显微注射纯化mRNA的非洲爪蟾卵母细胞产生的AChE来评估AChE mRNA。来自纤颤培养物和非纤颤培养物的mRNA产生的AChE分别为0.46和0.10 pmol乙酰胆碱水解·min⁻¹·卵母细胞⁻¹（P<0.005）。