Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan; Institute for Materials Chemistry and Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Colloids Surf B Biointerfaces. 2021 Mar;199:111517. doi: 10.1016/j.colsurfb.2020.111517. Epub 2020 Dec 8.
An excellent blood-compatible polymer, poly(2-methoxyethyl acrylate) (PMEA), exhibits nanometer-scale phase-separated structures at the interface with water or phosphate-buffered saline (PBS), and fibrinogen adsorption is suppressed, especially on the water-rich region. To understand the correlation between the interfacial structure based on the grafting density of PMEA and blood compatibility, grafted PMEA (gPMEA) surfaces with controlled density were prepared by immobilizing thiol-terminated PMEA on a gold substrate. The amount of adsorbed fibrinogen and the number of adhered platelets on gPMEAs decreased first with the increasing grafting density (σ), but increased after showed minimum at σ of approximately 0.11 chains/nm. The interfacial structures of the gPMEA/PBS interface changed with grafting density, and the maximum area of water-rich region was obtained at σ = 0.11. The water contact angle at σ = 0.11 is smaller than that at the other grafting density. These results revealed that hydration to the polymer is very effective to suppress the platelet adhesion and water-rich region shows excellent blood compatibility on gPMEA surfaces. This work clearly indicated that the density of PMEA affects the interfacial structure and plays an important role in the blood compatibility of the material.
一种优异的血液相容聚合物，聚（2-甲氧基乙基丙烯酸酯）（PMEA），在与水或磷酸盐缓冲盐水（PBS）的界面处呈现纳米级相分离结构，并且抑制纤维蛋白原吸附，特别是在富水区域。为了了解基于 PMEA 接枝密度的界面结构与血液相容性之间的相关性，通过将巯基封端的 PMEA 固定在金基底上来制备具有受控密度的接枝 PMEA（gPMEA）表面。吸附的纤维蛋白原量和粘附的血小板数在 gPMEAs 上随接枝密度（σ）的增加而先减少，但在约 0.11 链/nm 的 σ 后增加到最小值。gPMEA/PBS 界面的界面结构随接枝密度而变化，在 σ = 0.11 时获得富水区域的最大面积。在 σ = 0.11 时的水接触角小于其他接枝密度时的水接触角。这些结果表明，聚合物的水合作用对于抑制血小板粘附非常有效，并且富水区域在 gPMEA 表面上表现出优异的血液相容性。这项工作清楚地表明，PMEA 的密度会影响界面结构，并在材料的血液相容性中起重要作用。
