Refractories, Ceramics and Building Materials Department, National Research Centre, 33El Bohouth St. (Former EL Tahrir St.), Dokki, Giza P.O. 12622, Egypt.
Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 812-8582, Japan.
Int J Mol Sci. 2021 Jul 27;22(15):8038. doi: 10.3390/ijms22158038.
Intermediate water (IW) in hydrated bioactive glasses remains uninvestigated. We obtained titanium (Ti)-containing bioactive glasses (BGTs) (Ti at 5%, 7.5% and 10% of the glass system) using the sol-gel technique. Their thermal, physicochemical, and morphological properties, before and after Ti-doping, were analysed using DTA, XRD, FTIR, TEM, and SEM accessorised with EDAX, and size distribution and zeta potential surface charges were determined using a NanoZetasizer. The IW in hydrated BGTs was investigated by cooling and heating runs of DSC measurements. Moreover, the mode of death in an osteosarcoma cell line (MG63) was evaluated at different times of exposure to BGT discs. Ti doping had no remarkable effect on the thermal, physicochemical, and morphological properties of BGTs. However, the morphology, size, and charges of BGT nano-powders were slightly changed after inclusion of Ti compared with those of BGT0; for example, the particle size increased with increasing Ti content (from 4-5 to 7-28 nm). The IW content was enhanced in the presence of Ti. The mode of cell death revealed the effect of IW content on the proliferation of cells exposed to BGTs. These findings should help improve the biocompatibility of inorganic biomaterials.
水合生物活性玻璃中的中间水（IW）尚未得到研究。我们使用溶胶-凝胶技术获得了含有钛（Ti）的生物活性玻璃（BGT）（玻璃系统中 Ti 的含量为 5％、7.5％和 10％）。使用 DTA、XRD、FTIR、TEM 和 SEM 分析了它们在掺杂 Ti 前后的热、物理化学和形态特性，并使用带有 EDAX 的附件进行了尺寸分布和 ζ 电位表面电荷的测定，使用 NanoZetasizer 进行了测定。通过 DSC 测量的冷却和加热运行研究了水合 BGT 中的 IW。此外，还在不同的暴露时间评估了 Ti 掺杂对 BGT 盘的骨肉瘤细胞系（MG63）的死亡模式的影响。Ti 掺杂对 BGT 的热、物理化学和形态特性没有显著影响。然而，与 BGT0 相比，包含 Ti 后 BGT 纳米粉末的形态、尺寸和电荷略有变化；例如，粒径随 Ti 含量的增加而增加（从 4-5 纳米增加到 7-28 纳米）。IW 含量在 Ti 的存在下增加。细胞死亡模式揭示了 IW 含量对暴露于 BGT 的细胞增殖的影响。这些发现应该有助于提高无机生物材料的生物相容性。
