Wojtysiak Katarzyna, Ryszka Wojciech, Stefaniak Tadeusz, Król Jarosław, Kozdrowski Roland
Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366 Wroclaw, Poland.
Equi Salus, Glinno 65, 64-300 Nowy Tomyśl, Poland.
Animals (Basel). 2020 Dec 19;10(12):2438. doi: 10.3390/ani10122438.
The objective of the study was to evaluate the concentrations of interleukin-1 receptor antagonist (IL-1RA), interleukin-10 (IL-10), serum amyloid A (SAA) and haptoglobin (Hp) in uterine lavage fluid before and after artificial insemination (AI). Based on ultrasound examination, mares were divided into: Group 1 ( = 9), no fluid was detected in the uterus during estrus and 7 h after AI; Group 2 ( = 8), no fluid was detected in the uterus during estrus but 7 h after AI fluid was detected in the uterus; Group 3 ( = 8), fluid was detected in the uterus during estrus and also 7 h after AI. In all groups of mares, a significant increase in polymorphonuclear cells (PMN) and a significant increase in IL-1RA and SAA were recorded 7 h after AI. The obtained results show that, regardless of the status of the mare before AI, the endometrial response characterized by PMN influx, and SAA, Hp, IL-1RA and IL-10 production, is similar. The presence of intrauterine fluid during estrus is not connected with PMN influx but can impact uterine IL-1RA production at this time.
本研究的目的是评估人工授精（AI）前后子宫灌洗液中白细胞介素-1受体拮抗剂（IL-1RA）、白细胞介素-10（IL-10）、血清淀粉样蛋白A（SAA）和触珠蛋白（Hp）的浓度。根据超声检查，母马被分为：第1组（n = 9），发情期和人工授精后7小时子宫内未检测到液体；第2组（n = 8），发情期子宫内未检测到液体，但人工授精后7小时子宫内检测到液体；第3组（n = 8），发情期子宫内检测到液体，人工授精后7小时也检测到液体。在所有母马组中，人工授精后7小时记录到多形核细胞（PMN）显著增加，IL-1RA和SAA也显著增加。获得的结果表明，无论人工授精前母马的状态如何，以PMN流入以及SAA、Hp、IL-1RA和IL-10产生为特征的子宫内膜反应是相似的。发情期子宫内液体的存在与PMN流入无关，但此时可能影响子宫IL-1RA的产生。
