Ito Yoshihisa, Izawa Yusuke, Osaki Toshihisa, Kamiya Koki, Misawa Nobuo, Fujii Satoshi, Mimura Hisatoshi, Miki Norihisa, Takeuchi Shoji
Artificial Cell Membrane Systems Group, Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan.
School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
Micromachines (Basel). 2020 Dec 18;11(12):1123. doi: 10.3390/mi11121123.
Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection. In this paper, we propose a lipid-bilayer-on-a-cup device for serial sample measurements using a pumpless solution exchange procedure. The device consists of a millimeter-scale cylindrical cup with vertical slits which is designed to steadily hold an aqueous solution and exchange the sample by simply fusing and splitting the solution with an external solution. The slit design was experimentally determined by the capabilities of both the retention and exchange of the solution. Using the optimized slit, a planar lipid bilayer was reconstituted with a nanopore protein at a microaperture allocated to the bottom of the cup, and the device was connected to a portable amplifier. The solution exchangeability was demonstrated by observing the dilution process of a blocker molecule of the nanopore dissolved in the cup. The pumpless solution exchange by the proposed cup-like device presents potential as a lipid-bilayer system for portable sensing applications.
脂质双层器件已被用于诊断、食品和环境监测以及安全/安保检查等领域的现场传感器研究。在本文中，我们提出了一种用于连续样本测量的杯上脂质双层器件，该器件采用无泵溶液交换程序。该器件由一个带有垂直狭缝的毫米级圆柱形杯组成，其设计目的是稳定地容纳水溶液，并通过简单地将溶液与外部溶液融合和分离来交换样品。狭缝设计是通过溶液保留和交换能力的实验确定的。使用优化后的狭缝，在分配到杯底部的微孔处用纳米孔蛋白重构了平面脂质双层，并将该器件连接到便携式放大器。通过观察溶解在杯中纳米孔阻断分子的稀释过程，证明了溶液的交换能力。所提出的杯状器件进行的无泵溶液交换展现出作为便携式传感应用脂质双层系统的潜力。
