Nguyen Thu Yen Thi, Camboni Alessandra, Masciangelo Rossella, Donnez Jacques, Dolmans Marie-Madeleine
Gynecology Research Unit, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Av. Mounier 52, 1200 Brussels, Belgium.
Department of Anatomopathology, Cliniques Universitaires Saint-Luc, Av. Hippocrate 10, 1200 Brussels, Belgium.
J Clin Med. 2020 Dec 18;9(12):4101. doi: 10.3390/jcm9124101.
The risk of reseeding malignancy harbored in cryopreserved and transplanted ovarian tissue has been a source of concern. This study aimed to determine the potential relationship between frozen-thawed ovarian tissue transplantation and primary cancer recurrence. Three patients with cerebral primitive neuroectodermal tumors (PNET) were included in this study. One woman gave birth to three healthy babies following reimplantation of her cryopreserved ovarian tissue, but subsequently died due to cancer relapse six years after ovarian tissue transplantation. The second subject died from progressive cancer, while the third is still alive and awaiting reimplantation of her ovarian tissue in due course. Frozen ovarian cortex from all three patients was analyzed and xenotransplanted to immunodeficient mice for five months. Main outcomes were the presence of cancer cells in the thawed and xenografted ovarian tissue at histology, immunostaining (expression of neuron-specific enolase and glial fibrillary acidic protein (GFAP)), and reverse-transcription droplet digital polymerase chain reaction (RT-ddPCR) (levels of enolase 2 and GFAP). In conclusion, no malignant cells were detected in ovarian tissue from patients with PNET, even in those who experienced recurrence of the disease, meaning that the risk of reseeding cancer cells with ovarian tissue transplantation in these patients can be considered low.
冷冻保存并移植的卵巢组织中潜藏的恶性肿瘤再植风险一直备受关注。本研究旨在确定冻融卵巢组织移植与原发性癌症复发之间的潜在关系。本研究纳入了三名患有脑原始神经外胚层肿瘤（PNET）的患者。一名女性在重新植入其冷冻保存的卵巢组织后生下了三个健康的婴儿，但随后在卵巢组织移植六年后因癌症复发而死亡。第二名受试者死于癌症进展，而第三名受试者仍然存活，正在等待适时重新植入其卵巢组织。对所有三名患者的冷冻卵巢皮质进行了分析，并将其异种移植到免疫缺陷小鼠体内五个月。主要观察指标包括解冻和异种移植的卵巢组织在组织学、免疫染色（神经元特异性烯醇化酶和胶质纤维酸性蛋白（GFAP）的表达）以及逆转录液滴数字聚合酶链反应（RT-ddPCR）（烯醇化酶2和GFAP的水平）方面是否存在癌细胞。总之，在患有PNET的患者的卵巢组织中未检测到恶性细胞，即使在那些经历疾病复发的患者中也是如此，这意味着在这些患者中通过卵巢组织移植再植癌细胞的风险可被认为较低。
