Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York, USA.
Nat Struct Mol Biol. 2019 Sep;26(9):783-791. doi: 10.1038/s41594-019-0287-x. Epub 2019 Aug 22.
The landmark 1969 discovery of nuclear RNA polymerases I, II and III in diverse eukaryotes represented a major turning point in the field that, with subsequent elucidation of the distinct structures and functions of these enzymes, catalyzed an avalanche of further studies. In this Review, written from a personal and historical perspective, I highlight foundational biochemical studies that led to the discovery of an expanding universe of the components of the transcriptional and regulatory machineries, and a parallel complexity in gene-specific mechanisms that continue to be explored to the present day.
1969 年，在不同的真核生物中发现核 RNA 聚合酶 I、II 和 III，这是该领域的一个重要转折点。随着这些酶的结构和功能的进一步阐明，推动了大量进一步的研究。在这篇综述中，我从个人和历史的角度出发，重点介绍了基础生物化学研究，这些研究导致了转录和调控机制组件的扩展宇宙的发现，以及基因特异性机制的平行复杂性，这些机制至今仍在探索之中。
