真核转录：50 多年来的拓展与深化——不断涌现的因子与机制。
50+ years of eukaryotic transcription: an expanding universe of factors and mechanisms.
机构信息
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York, USA.
出版信息
Nat Struct Mol Biol. 2019 Sep;26(9):783-791. doi: 10.1038/s41594-019-0287-x. Epub 2019 Aug 22.
Abstract
The landmark 1969 discovery of nuclear RNA polymerases I, II and III in diverse eukaryotes represented a major turning point in the field that, with subsequent elucidation of the distinct structures and functions of these enzymes, catalyzed an avalanche of further studies. In this Review, written from a personal and historical perspective, I highlight foundational biochemical studies that led to the discovery of an expanding universe of the components of the transcriptional and regulatory machineries, and a parallel complexity in gene-specific mechanisms that continue to be explored to the present day.
摘要
1969 年，在不同的真核生物中发现核 RNA 聚合酶 I、II 和 III，这是该领域的一个重要转折点。随着这些酶的结构和功能的进一步阐明，推动了大量进一步的研究。在这篇综述中，我从个人和历史的角度出发，重点介绍了基础生物化学研究，这些研究导致了转录和调控机制组件的扩展宇宙的发现，以及基因特异性机制的平行复杂性，这些机制至今仍在探索之中。
相似文献
1
50+ years of eukaryotic transcription: an expanding universe of factors and mechanisms.
Nat Struct Mol Biol. 2019 Sep;26(9):783-791. doi: 10.1038/s41594-019-0287-x. Epub 2019 Aug 22.
2
The hunt for RNA polymerase II elongation factors: a historical perspective.
Nat Struct Mol Biol. 2019 Sep;26(9):771-776. doi: 10.1038/s41594-019-0283-1. Epub 2019 Aug 22.
3
A 50 year history of technologies that drove discovery in eukaryotic transcription regulation.
Nat Struct Mol Biol. 2019 Sep;26(9):777-782. doi: 10.1038/s41594-019-0288-9. Epub 2019 Aug 22.
4
The transformation of the DNA template in RNA polymerase II transcription: a historical perspective.
Nat Struct Mol Biol. 2019 Sep;26(9):766-770. doi: 10.1038/s41594-019-0278-y. Epub 2019 Aug 22.
5
Five decades of eukaryotic transcription.
Nat Struct Mol Biol. 2019 Sep;26(9):757. doi: 10.1038/s41594-019-0303-1.
6
Accidents and Damage Control.
Cell. 2015 Sep 10;162(6):1196-200. doi: 10.1016/j.cell.2015.08.042.
7
100 years of biochemistry and molecular biology. The decade-long pursuit of a reconstituted yeast transcription system: the work of Roger D. Kornberg.
J Biol Chem. 2009 Oct 23;284(43):e18-20.
8
Gcn5: The quintessential histone acetyltransferase.
Biochim Biophys Acta Gene Regul Mech. 2021 Feb;1864(2):194658. doi: 10.1016/j.bbagrm.2020.194658. Epub 2020 Nov 21.
9
Eukaryotic Transcription Turns 50.
Cell. 2019 Oct 31;179(4):808-812. doi: 10.1016/j.cell.2019.09.018.
10
Collective phenomena and the neglect of molecules: a historical outlook on biology.
Hist Philos Life Sci. 2007;29(1):83-6.
引用本文的文献
1
Pol III escapes the promoter using a "protein spring" mechanism.
Sci China Life Sci. 2025 Sep 5. doi: 10.1007/s11427-025-3028-x.
2
Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events.
bioRxiv. 2025 Aug 19:2025.08.18.670875. doi: 10.1101/2025.08.18.670875.
3
DSS1 is required for proper Integrator-PP2A function.
Nat Commun. 2025 Jul 5;16(1):6206. doi: 10.1038/s41467-025-61257-4.
4
Unraveling gene expression: a beginner's guide from chromatin modifications to mRNA export in .
Nucleus. 2025 Dec;16(1):2516909. doi: 10.1080/19491034.2025.2516909. Epub 2025 Jun 13.
5
The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcription.
iScience. 2025 May 5;28(6):112286. doi: 10.1016/j.isci.2025.112286. eCollection 2025 Jun 20.
6
Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips.
Plant Cell. 2025 Jul 1;37(7). doi: 10.1093/plcell/koaf137.
7
NDF/GLYR1 Promotes RNA Polymerase II Processivity via Pol II Binding and Nucleosome Destabilization.
Int J Mol Sci. 2025 May 19;26(10):4874. doi: 10.3390/ijms26104874.
8
RNA polymerase II partitioning is a shared feature of diverse oncofusion condensates.
Cell. 2025 Jul 10;188(14):3843-3862.e28. doi: 10.1016/j.cell.2025.04.002. Epub 2025 Apr 25.
9
Selective phase separation of transcription factors is driven by orthogonal molecular grammar.
Nat Commun. 2025 Mar 31;16(1):3087. doi: 10.1038/s41467-025-58445-7.
10
DNA-Protein Binding is Dominated by Short Anchoring Elements.
Adv Sci (Weinh). 2025 May;12(19):e2414823. doi: 10.1002/advs.202414823. Epub 2025 Mar 26.
本文引用的文献
1
Gene-Specific H1 Eviction through a Transcriptional Activator→p300→NAP1→H1 Pathway.
Mol Cell. 2019 Apr 18;74(2):268-283.e5. doi: 10.1016/j.molcel.2019.02.016. Epub 2019 Mar 19.
2
The role of transcription in shaping the spatial organization of the genome.
Nat Rev Mol Cell Biol. 2019 Jun;20(6):327-337. doi: 10.1038/s41580-019-0114-6.
3
Phase Separation, Protein Disorder, and Enhancer Function.
Cell. 2018 Dec 13;175(7):1723-1725. doi: 10.1016/j.cell.2018.11.034.
4
Structure of human TFIID and mechanism of TBP loading onto promoter DNA.
Science. 2018 Dec 21;362(6421). doi: 10.1126/science.aau8872. Epub 2018 Nov 15.
5
The many lives of KATs - detectors, integrators and modulators of the cellular environment.
Nat Rev Genet. 2019 Jan;20(1):7-23. doi: 10.1038/s41576-018-0072-4.
6
Organizational principles of 3D genome architecture.
Nat Rev Genet. 2018 Dec;19(12):789-800. doi: 10.1038/s41576-018-0060-8.
7
The Human Transcription Factors.
Cell. 2018 Oct 4;175(2):598-599. doi: 10.1016/j.cell.2018.09.045.
8
Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1.
Nat Struct Mol Biol. 2018 Sep;25(9):859-867. doi: 10.1038/s41594-018-0118-5. Epub 2018 Sep 6.
9
Emerging Roles of Non-Coding RNA Transcription.
Trends Biochem Sci. 2018 Sep;43(9):654-667. doi: 10.1016/j.tibs.2018.06.002. Epub 2018 Jun 28.
10
Structural basis of RNA polymerase III transcription initiation.
Nature. 2018 Jan 17;553(7688):301-306. doi: 10.1038/nature25441.
Zotero 插件